Coordinate changes in neuronal phenotype and surface antigen expression in human neuroblastoma cell variants Journal Article

  


Authors: Rettig, W. J.; Spengler, B. A.; Garin Chesa, P.; Old, L. J.; Biedler, J. L.
Article Title: Coordinate changes in neuronal phenotype and surface antigen expression in human neuroblastoma cell variants
Abstract: Human neuroblastoma cells growing in culture offer a unique opportunity to study proliferating human cells with a neuronal phenotype. We have previously identified several neuroblastoma cell lines which show spontaneous conversion (N/S interconversion) between two morphologically distinct cell types: neuroblastic (N-type) cells and variant, substrateadherent (S-type) cells resembling cultured glial or mesenchymal cells. In the present study, we have used molecular markers to confirm the neuronal phenotype of N-type cells and to demonstrate that S-type cells have a nonneuronal phenotype. Furthermore, we have used these markers, including a series of cell surface differentiation antigens, to compare S-type neuroblastoma cells with a wide range of cultured epithelial, mesenchymal, and neuroectodermal cells. The results suggest that (a) N/S interconversion represents an ordered transition between two neuroectodermal differentiation programs rather than random phenotypic instability of cultured cells; (b) S-type variant cells show a molecular phenotype most closely resembling the phenotype of cultured ectomesen-chymal cells; and (c) in vitro variant formation of human neuroblastomas may provide an experimental model for the observed in vivo transition of some malignant neuroblastomas into benign ganglioneuromas. © 1987, American Association for Cancer Research. All rights reserved.
Keywords: antigen expression; animal cell; phenotype; cytology; heredity; cell line; cell differentiation; neurons; receptor, epidermal growth factor; in vitro study; membrane antigen; antibodies, monoclonal; neuroblastoma; immunocytochemistry; neuroblastoma cell; antigens, surface; receptors, cell surface; nervous system; receptors, nerve growth factor; humans; human; priority journal
Journal Title: Cancer Research
Volume: 47
Issue: 5
ISSN: 0008-5472
Publisher: American Association for Cancer Research  
Date Published: 1987-03-01
Start Page: 1383
End Page: 1389
Language: English
PUBMED: 3028608
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023096977&partnerID=40&md5=4651e3e67f19145d9a37ea4c26166137
Notes: Article -- Export Date: 5 February 2021 -- Source: Scopus
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MSK Authors
  1. Lloyd J Old
    593 Old
  2. June Biedler
    83 Biedler
  3. Pilar Garin-Chesa
    73 Garin-Chesa
  4. Wolfgang J. Rettig
    85 Rettig
  5. Barbara Spengler
    44 Spengler
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