| Abstract: |
In human neuroblastoma cells in culture, three morphologically distinct types of cells are observed: neuroblastic N-type cells, Schwannian S-type cells, and intermediate I-type cells. To investigate the differences in gene expression between N-type LA1-55N and S-type LA1-5S cells of the human neuroblastoma cell line LA-N1, we constructed a subtractive cDNA library from LA1-5S cells. One of the genes that are expressed more in S-type cells than in N-type cells was identified as previously undescribed and is the focus of this report. We cloned a full-length cDNA of this gene, p37NB, and determined its sequence. A homology search against the GenBank database showed that this was from a novel gene encoding a putative 37 kDa leucine-rich repeat (LRR) protein. Northern blot hybridization and RT-PCR showed that the p37NB gene was differentially expressed in S-type compared to N-type cells of a few neuroblastoma cell lines. |
| Keywords: |
human cell; sequence analysis; proteins; gene expression; protein; cell differentiation; tumor cells, cultured; molecular cloning; cloning, molecular; gene expression regulation, neoplastic; amino acid sequence; molecular sequence data; cell culture; neuroblastoma; neuroblastoma cell; nucleotide sequence; base sequence; protein structure; leucine; blotting, northern; nucleotide repeat; complementary dna; gene structure; dna, complementary; consensus sequence; humans; human; priority journal; article; la-n-1; la1-55n; la1-5s; lrr protein; subtractive cdna library
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