Aberrant recombination events in B cell lines derived from a x-deficient human Journal Article
| Authors: | Stavnezer, J.; Kekish, O.; Batter, D.; Grenier, J.; Balazs, I.; Henderson, E.; Zegers, B. J. M. |
| Article Title: | Aberrant recombination events in B cell lines derived from a x-deficient human |
| Abstract: | We have analyzed the structure of Ig κ chain genes in B cell lines derived from a hiinan individual who cannot synthesize any κ chains, and whose Igs all contain λ chains (1). We have characterized secondary DNA recombination events at two κ alleles which have undergone misaligned V-J recombinations. One such secondary recombination has joined the flanking sequences of a Vκ and a Jκ2 gene segment as if it were the reciprocal product of a V-Jκ2 recombination, and resulted in the displacement of the recombined VJκ1 gene segments from the Cκ locus. The non-rearranged form of the Vκ fragment which had recombined with the Jκ2 flank was cloned. Nucleotide sequencing of this fragment identified a Vκ gene that differed by at least 38% from all previously sequenced human Vκ genes. The other V-Jκ segment analyzed has undergone a secondary recombination at a different site from that described above, at a site within the intervening sequence between the Jκ and Cκ gene segments, similar to the location of secondary recombinations which have occurred in λ + B cell lines from mice and humans (2, 3). These results prove that multiple recoiiibinations can occur at one Jκ-Cκ locus. © 1985 IRL Press Limited. |
| Keywords: | genetics; cell line; b lymphocyte; b-lymphocytes; molecular cloning; cloning, molecular; blood; genetic recombination; immunoglobulin variable region; recombination, genetic; nucleotide sequence; base sequence; immunoglobulin kappa chain; immunoglobulin light chain; human; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; dysgammaglobulinemia; immunoglobulins, light chain; immunoglobulins, light-chain; immunoglobulins, kappa chain; immunoglobulins, kappa-chain; immunoglobulin j chain; immunoglobulins, j chain; immunoglobulins, j-chain |
| Journal Title: | Nucleic Acids Research |
| Volume: | 13 |
| Issue: | 10 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 1985-05-24 |
| Start Page: | 3495 |
| End Page: | 3514 |
| Language: | English |
| DOI: | 10.1093/nar/13.10.3495 |
| PROVIDER: | scopus |
| PMCID: | PMC341255 |
| PUBMED: | 3925437 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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