Increased chromosomal instability in lymphocytes from elderly humans Journal Article
| Authors: | Dutkowski, R. T.; Lesh, R.; Staiano-Coico, L.; Thaler, H.; Darlington, G. J.; Weksler, M. E. |
| Article Title: | Increased chromosomal instability in lymphocytes from elderly humans |
| Abstract: | Lymphocytes from young and old donors were incubated with PHA for 96 h and exposed to [3H]Tdr during the last 24 h of culture. Comparable amounts of [3H]Tdr were incorporated into chromosomes of old and young lymphocytes as measured by autoradiography of metaphase chromosomes. However, chromosomal damage and cell-cycle arrest were far greater in lymphocytes from old as compared to young humans. The frequency of chromosome breaks, fragments, exchange figures and dicentric chromosomes induced by [3H]Tdr was greater in cultures from old than in cultures from young humans. Lymphocytes from old donors exposed to 20 μM BrdU during the last 24 h of culture showed significantly more sister-chromatid exchanges than did lymphocytes from young donors. These data suggest that chromosomes in lymphocytes from old donors express more damage after exposure to [3H]Tdr or BrdU than do chromosomes in lymphocytes from young donors. © 1985. |
| Keywords: | adult; aged; unclassified drug; human cell; chromosome; cell cycle; dna repair; in vitro study; histology; age; lymphatic system; sister chromatid exchange; aging; radioisotope; chromosome aberrations; lymphocyte; lymphocytes; chromosomes; normal human; thymidine; broxuridine; thymidine h 3; tritium; instability; etiology; humans; human; priority journal; article; blood and hemopoietic system |
| Journal Title: | Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis |
| Volume: | 149 |
| Issue: | 3 |
| ISSN: | 0027-5107 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1985-05-01 |
| Start Page: | 505 |
| End Page: | 512 |
| Language: | English |
| DOI: | 10.1016/0027-5107(85)90169-1 |
| PROVIDER: | scopus |
| PUBMED: | 3990698 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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