Stereoisomeric configuration of arabinitol in serum, urine, and tissues in invasive candidiasis Journal Article
| Authors: | Bernard, E. M.; Wong, B.; Armstrong, D. |
| Article Title: | Stereoisomeric configuration of arabinitol in serum, urine, and tissues in invasive candidiasis |
| Abstract: | Because routine analytical methods cannot differentiate D- from L-arabinitol, a combined microbiological and gas chromatographic method was developed to study the stereoisomeric configuration of the arabinitol in humans and rats with invasive candidiasis. D-Arabinitol was defined as the difference between arabinitol concentrations measured with and without incubation with 5.0 x 105 blastospores of Candida tropicalis strain CT 12 at 37 C for 24 hr. The yeast consumed at least 95% of the D-arabinitol and none of the L-arabinitol added to normal serum and urine. D-Arabinitol as a fraction of D,L-arabinitol was 0.43 ± 0.15 (mean ± SD) in the urine of 10 normal humans, 0.82 ± 0.12 in the serum or urine of five patients with cancer and invasive candidiasis (P < .001), and 1.0 in the kidneys of rats with candidiasis. Because most or all of the excess arabinitol in body fluids or tissues in candidiasis was the D isomer, which is produced by fungal metabolism, stereospecific quantitation of arabinitol should improve the sensitivity of this approach to diagnosis of candidiasis. |
| Keywords: | creatinine; kidney; diagnosis; rat; drug tissue level; urine; drug blood level; drug determination; serum; candidiasis; fungus; drug urine level; cancer; human; priority journal; drug analysis; arabinitol |
| Journal Title: | Journal of Infectious Diseases |
| Volume: | 151 |
| Issue: | 4 |
| ISSN: | 0022-1899 |
| Publisher: | Oxford University Press |
| Date Published: | 1985-04-01 |
| Start Page: | 711 |
| End Page: | 715 |
| Language: | English |
| DOI: | 10.1093/infdis/151.4.711 |
| PUBMED: | 3973418 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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