Characterization and cell cycle kinetics of hepatocyte populations isolated from adult liver tissue by a nonenzymatic procedure Journal Article

  

Authors:	Higgins, P. J.; Kessler, G. K.; Nissebaum, J. S.; Melamed, M. R.
Article Title:	Characterization and cell cycle kinetics of hepatocyte populations isolated from adult liver tissue by a nonenzymatic procedure
Abstract:	Suspensions of liver cells enriched in lobular parenchymal hepatocytes were isolated from adult mouse hepatic tissue by nonenzymatic dispersion in chelating buffer and sedimentation of the released cells at unit g. Single cell suspensions so obtained were suitable for flow cytometric measurements of hepatic ploidy class distributions. The more quickly sedimenting cell population consisted of 88% albumin/transferrin-positive epithelial hepatocytes, the nuclei of which were bimodally distributed with respect to RNA content. This dual G1 population was observed in 2C DNA content liver nuclei prepared by several methods and appears to be a general cytochemical characteristic of adult liver parenchymal cells.
Keywords:	nonhuman; flow cytometry; methodology; animal cell; mouse; cell cycle; in vitro study; animalia; liver; liver cell; ploidy; priority journal
Journal Title:	Journal of Histochemistry and Cytochemistry
Volume:	33
Issue:	7
ISSN:	0022-1554
Publisher:	Sage Publications
Date Published:	1985-07-01
Start Page:	672
End Page:	676
Language:	English
DOI:	10.1177/33.7.2409127
PROVIDER:	scopus
PUBMED:	2409127
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021856358&doi=10.1177%2f33.7.2409127&partnerID=40&md5=7be1d89fbe72ab3d9bd98c1c4443de43
Notes:	Article -- Export Date: 26 October 2021 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/189885