Analysis of cell surface light chain immunoglobulin expression by flow cytometry in normal controls: A new mathematical approach Journal Article

  

Authors:	Wong, G. Y.; Gebhard, D.; Mittleman, A.; Hancu, M.; Koziner, B.
Article Title:	Analysis of cell surface light chain immunoglobulin expression by flow cytometry in normal controls: A new mathematical approach
Abstract:	It has been observed in flow cytometric studies that in normal individuals there are proportionally more kappa+ than lamba+ bearing light chain B-cells. Overt predominance of one type of light bearing cell over the other is a characteristic of B-cell neoplasias, a phenomenon called clonal excess (CE). A mathematical model using the Weibull distribution is proposed for studying such an excess. The new approach is desirable for two reasons: First, it is parametric and hence offers a more sensitive and versatile analysis than its nonparametric counterparts. Second, it utilizes only the relevant information from the upper tails of the distributions of the fluorescence intensity of the kappa+ and lambda+ cells. Two measures of CE based on the Weibull model are proposed, and a normal range of variability was determined for each measure using a random sample of 48 normal controls. Such normal ranges are particularly useful in detecting cancer patients with minimal B-cell neoplasias. A comparative study of the new measures, Ault's maximum difference measure, and a measure based on Ligler's method showed that the parametric approach provides much more sensitivity than both the nonparametric ones.
Keywords:	human cell; flow cytometry; methodology; cytology; b lymphocyte; mathematical model; lymphatic system; diagnosis; cell membrane; lymphoma; lymphatic leukemia; immunoglobulin light chain; normal human; cell surface; nonbiological model; human; priority journal; blood and hemopoietic system
Journal Title:	Journal of Histochemistry and Cytochemistry
Volume:	33
Issue:	2
ISSN:	0022-1554
Publisher:	Sage Publications
Date Published:	1985-02-01
Start Page:	119
End Page:	126
Language:	English
DOI:	10.1177/33.2.3918096
PROVIDER:	scopus
PUBMED:	3918096
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021967605&doi=10.1177%2f33.2.3918096&partnerID=40&md5=6a70241e9083521ab80328b9107da724
Notes:	Article -- Export Date: 26 October 2021 -- Source: Scopus

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