Immunopathologic analysis of a panel of mouse monoclonal antibodies reacting with human ovarian carcinomas and other human tumors Journal Article

  


Authors: Cordon-Cardo, C.; Mattes, M. J.; Melamed, M. R.; Lewis, J. L. Jr; Old, L. J.; Lloyd, K. O.
Article Title: Immunopathologic analysis of a panel of mouse monoclonal antibodies reacting with human ovarian carcinomas and other human tumors
Abstract: The reactivity of a panel of five mouse monoclonal antibodies (mAb) produced to human ovarian and endometrial cancer cell lines was analyzed on frozen sections of normal adult and fetal tissues and on tumor sections using immunofluorescence and immunoperoxidase procedures. mAb MH99 was reactive with almost all types of epithelial cells and stained all epithelial tumors examined. mAb MH94 reacted mainly with secretory epithelia and was unreactive with the epithelial cell layer of normal ovaries. In contrast, approximately 50% of epithelial ovarian tumors and the lining cells of inclusion cysts were positive. mAb MF61 was positive only on the endometrium and noncellular follicular secretions or colloid of the thyroid among normal tissues examined. A subset of ovarian tumors was positive with this antibody. mAb MF116 gave no reactivity with normal tissues within the sensitivity of the assay. MF116 was expressed on a proportion (44%) of ovarian carcinomas of various histological types. These antibodies provide an initial panel for the immunohistological analysis of ovarian cancer. © 1985 International Society of Gynecological Pathologists.
Keywords: immunohistochemistry; ovarian neoplasms; mouse; cytology; animals; mice; cell line; monoclonal antibodies; monoclonal antibody; immunoenzyme techniques; fluorescent antibody technique; antibodies, monoclonal; antigens, neoplasm; diagnosis; ovary carcinoma; female genital system; endocrine system; humans; human; female; ovarian tumors
Journal Title: International Journal of Gynecological Pathology
Volume: 4
Issue: 2
ISSN: 0277-1691
Publisher: Lippincott Williams & Wilkins  
Date Published: 1985-06-01
Start Page: 121
End Page: 130
Language: English
DOI: 10.1097/00004347-198506000-00004
PROVIDER: scopus
PUBMED: 3894261
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0021845058&doi=10.1097%2f00004347-198506000-00004&partnerID=40&md5=a965a48ea703fd3a915d5f9caf5e0294
Notes: Article -- Export Date: 26 October 2021 -- Source: Scopus
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MSK Authors
  1. Carlos Cordon-Cardo
    612 Cordon-Cardo
  2. Lloyd J Old
    593 Old
  3. Kenneth O Lloyd
    163 Lloyd
  4. John Lewis
    132 Lewis
  5. Myron Melamed
    148 Melamed
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