Class II positive human dermal fibroblasts restimulate cloned allospecific T cells but fail to stimulate primary allogeneic lymphoproliferation Journal Article
| Authors: | Maurer, D. H.; Collins, W. E.; Hanke, J. H.; Van, M.; Rich, R. R.; Pollack, M. S. |
| Article Title: | Class II positive human dermal fibroblasts restimulate cloned allospecific T cells but fail to stimulate primary allogeneic lymphoproliferation |
| Abstract: | Recently, a number of laboratories have shown that gamma interferon (IFN-γ) is a potent modulator of HLA class II antigen expression in a variety of cell types ranging from classical antigen presenting cells to those not expected to participate in physiological antigen presentation such as fibroblasts. In order to examine the role of HLA class II expressing fibroblasts in antigen presentation, we established dermal fibroblast (FIB) strains from five HLA typed donors. After optimal preculture with IFN-γ, class II positive FIB were fully competent to restimulate proliferative responses of two DR specific T cell clones and one DP specific T cell line. However, they failed to elicit strong primary allogeneic proliferation from fully DR mismatched fresh PBMC. This failure was not due to a direct suppressive effect of FIB and could not be corrected by exogenous IL1 or by factors contained in conventional mixed leukocyte culture supernatants. © 1985. |
| Keywords: | human cell; t lymphocyte; cells, cultured; heredity; biological model; in vitro study; skin; antigen presentation; lymphocyte activation; gamma interferon; hla antigen class 2; fibroblast; fibroblasts; histocompatibility antigens class ii; antigen-presenting cells; immunologic memory; interleukin-1; lymphocyte transformation; interferon type ii; humans; human; priority journal; blood and hemopoietic system |
| Journal Title: | Human Immunology |
| Volume: | 14 |
| Issue: | 3 |
| ISSN: | 0198-8859 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1985-11-01 |
| Start Page: | 245 |
| End Page: | 258 |
| Language: | English |
| DOI: | 10.1016/0198-8859(85)90232-0 |
| PUBMED: | 3932267 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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