Surface immunoglobulin light-chain expression by the "common" all cell line REH Journal Article
| Authors: | Koziner, B.; Stavnezer, J.; Al-Katib, A. |
| Article Title: | Surface immunoglobulin light-chain expression by the "common" all cell line REH |
| Abstract: | To provide further support of the B-cell lineage of "common" acute lymphoblastic leukemia (ALL), we studied the REH cell line and found not only cytoplasmic heavy chain but also surface λ light-chain expression by flow cytometry. Nucleic acid blotting experiments on REH revealed that both κ genes had been deleted and that λ genes had been rearranged, as expected in B cells expressing λ light chain. Moreover, REH cells contained μ and λ RNA. When REH cells were treated with TPA the amount of μ chain RNA increased by approximately fivefold and the amount of λ chain RNA increased by approximately twofold. The finding of surface immunoglobulin λ light chain in the REH cell line, suggests that these leukemic cells are further differentiated along the B-cell lineage than was previously believed. © 1985. |
| Keywords: | flow cytometry; antigen expression; phenotype; cell line; cell differentiation; in vitro study; acute lymphoblastic leukemia; b lymphocyte; b-lymphocytes; lymphatic system; rna; immunoglobulin light chain; cell separation; lymphocyte antibody; nucleic acid; tetradecanoylphorbol acetate; leukemia, lymphocytic; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; immunoglobulins, light-chain; blood and hemopoietic system; immunoglobulins, surface |
| Journal Title: | Clinical Immunology and Immunopathology |
| Volume: | 37 |
| Issue: | 1 |
| ISSN: | 0090-1229 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1985-10-01 |
| Start Page: | 135 |
| End Page: | 141 |
| Language: | English |
| DOI: | 10.1016/0090-1229(85)90144-8 |
| PUBMED: | 2411455 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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