Cell-type-specific synthesis of murine immunoglobulin μ RNA from an adenovirus vector Journal Article
| Authors: | Ruether, J. E.; Maderious, A.; Lavery, D.; Logan, J.; Fu, S. M.; Chen-Kiang, S. |
| Article Title: | Cell-type-specific synthesis of murine immunoglobulin μ RNA from an adenovirus vector |
| Abstract: | The mouse immunoglobulin heavy-chain μ constant region gene was cloned into the early region 1B of an adenovirus type 5 vector to allow reproducible kinetics of expression of the μ gene in the presence of continuous host protein synthesis after infection by the recombinant. The immunoglobulin-adenovirus recombinant is helper independent in infecting human fibroblastic and B- and T-cell lines and expresses μ in a cell-type-specific manner. By Northern blot analysis, correctly polyadenylated and spliced E1B-μ(s) and E1BmRNAs are found to be equally abundant at steady state in fibroblasts. In contrast, and appropriately, only E1B-μ(s) mRNAs accumulate in a lambda light-chain-secreting myeloma cell line. Analysis of nascent transcripts pulse labeled in isolated nuclei demonstrates equimolar polymerase loading throughout the μ region in all cell types infected by μ-Ad. Thus, correct polyadenylation and splicing of E1B-μ(s) and E1B-μ(m) in fibroblasts does not require transcription termination in the region separating the μ(s) and μ(m) polyadenylation sites. Furthermore, differential expression of μ transcripts in the background of myeloma cells is regulated at the level of RNA processing and does not require the presence of the immunoglobulin heavy-chain enhancer or promoter element. |
| Keywords: | human cell; genetics; t lymphocyte; t-lymphocytes; mouse; animal; mice; heredity; gene expression; cell line; genetic transcription; transcription, genetic; in vitro study; b lymphocyte; b-lymphocytes; genetic vectors; molecular cloning; cloning, molecular; immunology; immunoglobulin gene; genetic engineering; cell culture; messenger rna; rna, messenger; plasmid; murinae; plasmids; virus infection; radioisotope; virus recombinant; adenoviridae; immunoglobulin m; immunoglobulin mu chain; dna vector; adenoviruses, human; human adenovirus type 5; adenovirus 5; human; article; support, u.s. gov't, p.h.s.; immunoglobulin constant region; immunoglobulins, mu-chain; human adenovirus; immunoglobulins, mu chain |
| Journal Title: | Molecular and Cellular Biology |
| Volume: | 6 |
| Issue: | 1 |
| ISSN: | 0270-7306 |
| Publisher: | American Society for Microbiology |
| Date Published: | 1986-01-01 |
| Start Page: | 122 |
| End Page: | 133 |
| Language: | English |
| PUBMED: | 3097501 |
| PROVIDER: | scopus |
| PMCID: | PMC367491 |
| DOI: | 10.1128/mcb.6.1.123-133.1986 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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