Organization and chromosomal specificity of autosomal homologs of human Y chromosome repeated DNA Journal Article
| Authors: | Burk, R. D.; Szabo, P.; O'Brien, S.; Nash, W. G.; Yu, L.; Smith, K. D. |
| Article Title: | Organization and chromosomal specificity of autosomal homologs of human Y chromosome repeated DNA |
| Abstract: | The human Y chromosome contains a group of repeated DNA elements, identified as 3.4-kilobase pair (kb) fragments in Hae III digests of male genomic DNA, which contain both Y-specific and non-Y-specific sequences. We have used these 3.4-kb Hae III Y fragments to explore the organizational properties and chromosomal distribution of the autosomal homologs of the non-Y-specific (NYS) 3.4-kb Hae III Y elements. Three distinct organizations, termed domains, have been identified and shown to have major concentrations on separate chromosomes. We have established that domain K is located on chromosome 15 and domain D on chromosome 16 and suggested that domain R is on chromosome 1. Our findings suggest that each domain is composed of a tandemly arrayed cluster of a regularly repeating unit containing two sets of repeated sequences: one that is homologous to the NYS 3.4-kb Hae III Y sequences and one that does not cross-react with the 3.4-kb Hae III Y repeats. Thus, these autosomal repeated DNA domains, like their Y chromosome counterparts, consist of a complex mixture of repeated DNA elements interspersed among each other in ways that lead to defined periodicities. Although each of the three identified autosomal domains cross-reacts with 3.4-kb Hae III Y fragments purified from genomic DNA, the length periodicities and sequence content of the autosomal domains are chromosome specific. The organizational properties and chromosomal distribution of these NYS 3.4-kb Hae III homologs seem inconsistent with stochastic mechanisms of sequence diffusion between chromosomes. © 1985 Springer-Verlag. |
| Keywords: | human cell; animals; heredity; cell line; histology; dna; genetic engineering; cell nucleus; radioisotope; nucleic acid hybridization; sex factors; autosome; molecular weight; repetitive sequences, nucleic acid; normal human; y chromosome; cricetinae; cricetulus; chromosome 16; hybrid cells; repetitive dna; chromosome 15; normal value; humans; human; male; female; dna restriction enzymes; dna probe |
| Journal Title: | Chromosoma |
| Volume: | 92 |
| Issue: | 3 |
| ISSN: | 0009-5915 |
| Publisher: | Springer |
| Date Published: | 1985-06-01 |
| Start Page: | 225 |
| End Page: | 233 |
| Language: | English |
| DOI: | 10.1007/bf00348698 |
| PUBMED: | 2990828 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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