The monoclonal antibodies αS-HCL 1 (αLeu-14) and αS-HCL 3 (αLeu-M5) allow the diagnosis of hairy cell leukemia Journal Article
| Authors: | Schwarting, R.; Stein, H.; Wang, C. Y. |
| Article Title: | The monoclonal antibodies αS-HCL 1 (αLeu-14) and αS-HCL 3 (αLeu-M5) allow the diagnosis of hairy cell leukemia |
| Abstract: | To define cell surface antigens associated with hairy cell leukemia (HCL), and to gain better insight into the origin of this disease, we developed monoclonal antibodies against spleen cells of a patient with this disease. Although none of these antibodies alone proved specific for the leukemic cells, two of them, designated αS-HCL 1 (αLeu-14) and αS-HCL 3 (Λeu-M5) were found to be valuable in the diagnosis of HCL when used in combination. αS-HCL 1 recognizes an antigen associated with >95% of B cells in the peripheral blood. Biochemical analysis identified this antigen as a single polypeptide chain with a molecular weight of 150,000 dalton (150 kilodaltons). Σ-HCL 1 expression on hairy cells is markedly increased when compared with normal B lymphocytes isolated from peripheral blood, tonsils, and spleens. αS-HCL 3 reacts with an antigen present on hairy cells but also on monocytes, macrophages, in a lower density on neutrophils, and a small percentage (<2%) of lymphocytes. The antigen recognized by Σ-HCL 3 is composed of a non-covalently linked biomolecular complex of 90 and 150 kilodaltons. Since the HCL 3 antigen was not detectable on other lymphomas of either T or B cell type, the co-expression of S-HCL 1 and S-HCL 3 on hairy cells is a unique marker for this disease. |
| Keywords: | clinical article; monoclonal antibody; lymphatic system; membrane antigen; hairy cell leukemia; human; priority journal; blood and hemopoietic system |
| Journal Title: | Blood |
| Volume: | 65 |
| Issue: | 4 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 1985-04-01 |
| Start Page: | 974 |
| End Page: | 983 |
| Language: | English |
| DOI: | 10.1182/blood.v65.4.974.974 |
| PUBMED: | 3978236 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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