Phorbol ester‐induced hairy cell features on chronic lymphocytic leukemia cells in vitro Journal Article
| Authors: | Ai‐Katib, A.; Wang, C. Y.; McKenzie, S.; Clarkson, B. D.; Koziner, B. |
| Article Title: | Phorbol ester‐induced hairy cell features on chronic lymphocytic leukemia cells in vitro |
| Abstract: | Leukemic cells from eight patients with chronic lymphocytic leukemia were isolated and cultured in the continuous presence of 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) at a concentration of 1.6 × 10−9 M for 4–10 days. Aliquots of cells were then analyzed at intervals of 24–72 hr for changes in morphology, acid phosphatase staining (AP), and expression of two hairy cell‐associated surface antigens, HCL1 (CD22, Leu 14) and HCL3 (CD11c, Leu M5). All cases studied showed typical B‐CLL phenotype, and only a small proportion of cells expressed CD22 and CD11c (mean 7% and 4.9%, respectively). TPA treatment induced the coexpression of CD22 (mean 49%) and CD11c (mean 48%) and tartrate‐resistant acid phosphatase in seven of eight cases. Morphologically, cells in TPA cultures expressed hairy cell features that were evident in light and electron microscopic studies. Collectively these changes indicate that TPA can induce hairy cell features on CLL cells in vitro, suggesting the later maturational stage of HCL compared with CLL. © 1992 Wiley‐Liss, Inc. Copyright © 1992 Wiley‐Liss, Inc., A Wiley Company |
| Keywords: | adult; clinical article; aged; human cell; phenotype; electron microscopy; microscopy, electron; dose-response relationship, drug; time factors; membrane antigen; leukemia cell; antigens, cd; chronic lymphatic leukemia; lymphocytes; cll; phorbol esters; electrophoresis, polyacrylamide gel; hairy cell leukemia; phorbol 13 acetate 12 myristate; tetradecanoylphorbol acetate; acid phosphatase; middle age; leukemia, lymphocytic, chronic; antigens, differentiation, b-lymphocyte; tpa; leukemia, hairy cell; precipitin tests; antigens, cd11; human; male; female; priority journal; article |
| Journal Title: | American Journal of Hematology |
| Volume: | 40 |
| Issue: | 4 |
| ISSN: | 0361-8609 |
| Publisher: | John Wiley & Sons, Inc. |
| Date Published: | 1992-08-01 |
| Start Page: | 264 |
| End Page: | 269 |
| Language: | English |
| DOI: | 10.1002/ajh.2830400405 |
| PUBMED: | 1354412 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 30 July 2019 -- Source: Scopus |
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