Tracer priming in human protein turnover studies with [(15)N] glycine Journal Article
| Authors: | Jeevanandam, M.; Brennan, M. F.; Horowitz, G. D.; Rose, D.; Mihranian, M. H.; Daly, J.; Lowry, S. F. |
| Article Title: | Tracer priming in human protein turnover studies with [(15)N] glycine |
| Abstract: | Sixty-three studies in healthy normal volunteers (n = 29), malnourished cancer (n = 8) or non-cancer patients (n = 9), and postoperative radical cystectomy patients (n = 17) were conducted to evaluate the primed constant infusion labeling technique for the estimation of whole-body protein turnover under a variety of dietary conditions. [15N]Glycine was used as the tracer with a prime to infusion ratio of 1300 to 3300 min and a continuous-infusion rate of 0.11 to 0.33 μg 15N · kg-1 · min-1 for 24 to 36 hr. The isotopic steady-state enrichment was reached in all subjects both in urinary urea and ammonia between 10 and 26 hr (mean 18 ± 2). During protein calorie fasting the attainment of isotopic steady state is much quicker (10 to 18 hr) with a primed constant infusion than with a constant infusion alone (≈38 hr). A P I ratio greater or less than 1800 (min) usually resulted in a delay of plateau attainment without affecting the protein turnover values. Reliable estimates of protein kinetics in humans can be made in clinical conditions with a 26-hr infusion of glycine at the rate of 0.28 μg 15N · kg-1 · min-1 with a P I ratio of 1800 min, collecting six urine samples every 2 hr from 16 hr and analyzing for both urinary urea and ammonia enrichments. © 1985. |
| Keywords: | clinical article; unclassified drug; neoplasms; proteins; protein metabolism; kinetics; metabolic clearance rate; urine; malnutrition; infusions, parenteral; glycine; nitrogen; injections, intravenous; nitrogen isotopes; mathematics; ammonia; cancer; humans; human; priority journal; article; nutrition disorders; glycine n 15 |
| Journal Title: | Biochemical Medicine |
| Volume: | 34 |
| Issue: | 2 |
| ISSN: | 0006-2944 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1985-10-01 |
| Start Page: | 214 |
| End Page: | 225 |
| Language: | English |
| DOI: | 10.1016/0006-2944(85)90114-0 |
| PUBMED: | 4084245 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
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38Jeevanandam
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