Characterization, isolation, and in vitro culture of leptomeningeal fibroblasts Journal Article


Authors: Remsik, J.; Saadeh, F.; Tong, X.; Li, M. J.; Snyder, J.; Bale, T.; Wu, J.; Derderian, C.; Guber, D.; Chi, Y.; Murali, R.; Boire, A.
Article Title: Characterization, isolation, and in vitro culture of leptomeningeal fibroblasts
Abstract: Meninges, or the membranous coverings of the brain and spinal cord, play host to dozens of morbid pathologies. In this study we provide a method to isolate the leptomeningeal cell layer, identify leptomeninges in histologic slides, and maintain leptomeningeal fibroblasts in in vitro culture. Using an array of transcriptomic, histological, and cytometric analyses, we identified ICAM1 and SLC38A2 as two novel markers of leptomeningeal cells in vivo and in vitro. Our results confirm the fibroblastoid nature of leptomeningeal cells and their ability to form a sheet-like layer that covers the brain and spine parenchyma. These findings will enable researchers in central nervous system barriers to describe leptomeningeal cell functions in health and disease. © 2021 Elsevier B.V.
Journal Title: Journal of Neuroimmunology
Volume: 361
ISSN: 0165-5728
Publisher: Elsevier Inc.  
Date Published: 2021-12-15
Start Page: 577727
Language: English
DOI: 10.1016/j.jneuroim.2021.577727
PROVIDER: scopus
PUBMED: 34688068
PMCID: PMC8648257
DOI/URL:
Notes: Article -- Export Date: 2 November 2021 -- Source: Scopus
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MSK Authors
  1. Rajmohan Murali
    219 Murali
  2. Adrienne Boire
    106 Boire
  3. Tejus Bale
    122 Bale
  4. Yudan Chi
    6 Chi
  5. Jan Remsik
    25 Remsik
  6. David Guber
    3 Guber
  7. Fadi Saadeh
    4 Saadeh
  8. Xinran Tong
    6 Tong
  9. Min Jun Li
    6 Li
  10. Jenna M Snyder
    4 Snyder
  11. Jean Wu
    1 Wu