Synapse - Mesonephric and mesonephric-like carcinomas of the female genital tract: Molecular characterization including cases with mixed histology and matched metastases

Mesonephric and mesonephric-like carcinomas of the female genital tract: Molecular characterization including cases with mixed histology and matched metastases Journal Article

Authors:	da Silva, E. M.; Fix, D. J.; Sebastiao, A. P. M.; Selenica, P.; Ferrando, L.; Kim, S. H.; Stylianou, A.; Da Cruz Paula, A.; Pareja, F.; Smith, E. S.; Zehir, A.; Konner, J. A.; Cadoo, K.; Reis-Filho, J. S.; Abu-Rustum, N. R.; Mueller, J. J.; Weigelt, B.; Park, K. J.
Article Title:	Mesonephric and mesonephric-like carcinomas of the female genital tract: Molecular characterization including cases with mixed histology and matched metastases
Abstract:	Mesonephric carcinoma of the cervix is a rare tumor derived from Wolffian remnants. Mesonephric-like carcinomas of the ovary and endometrium, while morphologically similar, do not have obvious Wolffian derivation. Here, we sought to characterize the repertoire of genetic alterations in primary mesonephric and mesonephric-like carcinomas, in the distinct histologic components of mixed cases, as well as in matched primary tumors and metastases. DNA from microdissected tumor and normal tissue from mesonephric carcinomas (cervix, n = 8) and mesonephric-like carcinomas (ovarian n = 15, endometrial n = 13) were subjected to sequencing targeting 468 cancer-related genes. The histologically distinct components of four cases with mixed histology and four primary tumors and their matched metastases were microdissected and analyzed separately. Mesonephric-like carcinomas were underpinned by somatic KRAS mutations (25/28, 89%) akin to mesonephric carcinomas (8/8, 100%), but also harbored genetic alterations more frequently reported in Müllerian tumors. Mesonephric-like carcinomas that lacked KRAS mutations harbored NRAS (n = 2, ovary) or BRAF (n = 1, endometrium) hotspot mutations. PIK3CA mutations were identified in both mesonephric-like (8/28, 28%) and mesonephric carcinomas (2/8, 25%). Only mesonephric-like tumors harbored CTNNB1 hotspot (4/28, 14%) and PTEN (3/13, 23%) mutations. Copy number analysis revealed frequent gains of chromosomes 1q and 10 in both mesonephric (87% 1q; 50% chromosome 10) and mesonephric-like tumors (89% 1q; 43% chromosome 10). Chromosome 12 gains were more frequent in ovarian mesonephric-like carcinomas, and losses of chromosome 9 were more frequent in mesonephric than in mesonephric-like carcinomas (both p = 0.01, Fisher’s exact test). The histologically distinct components of four mixed cases were molecularly related and shared similar patterns of genetic alterations. The progression from primary to metastatic lesions involved the acquisition of additional mutations, and/or shifts from subclonal to clonal mutations. Our findings suggest that mesonephric-like carcinomas are derived from a Müllerian substrate with differentiation along Wolffian/mesonephric lines. © 2021, The Author(s), under exclusive licence to United States & Canadian Academy of Pathology.
Journal Title:	Modern Pathology
Volume:	34
Issue:	8
ISSN:	0893-3952
Publisher:	Nature Research
Date Published:	2021-08-01
Start Page:	1570
End Page:	1587
Language:	English
DOI:	10.1038/s41379-021-00799-6
PUBMED:	33772212
PROVIDER:	scopus
PMCID:	PMC8343944
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-85103350158&doi=10.1038%2fs41379-021-00799-6&partnerID=40&md5=633d6a107d01c9e86c4ef49674d2b7f3
Notes:	Article -- Export Date: 1 September 2021 -- Source: Scopus