Polyproteins containing a domain encoded by the V-SKI oncogene are located in the nuclei of SKV-transformed cells Journal Article
| Authors: | Barkas, A. E.; Brodeur, D.; Stavnezer, E. |
| Article Title: | Polyproteins containing a domain encoded by the V-SKI oncogene are located in the nuclei of SKV-transformed cells |
| Abstract: | SKV-transformed nonproducer clones were isolated from infected quail and chicken embryo cells. Analysis of intracellular viral RNAs by the Northern technique revealed that each clone contained a single SKV genome (either 5.7 or 8.9 kb) but no genome of the helper virus. Analysis of intracellular viral proteins containing gag determinants revealed that each clone contained a single species of either 55, 110, or 125 kDa. The intracellular location of these proteins was determined by indirect immunofluorescence employing either monoclonal antibodies (anti-p19gag) or conventional antiserum against gag proteins. All three of the SKV-specific proteins were localized to the nuclei of the transformed cells. © 1986. |
| Keywords: | nonhuman; animal; cells, cultured; immunofluorescence; in vitro study; avian leukosis oncovirus; cell transformation, neoplastic; oncogenes; monoclonal antibody; oncogene; fluorescent antibody technique; cell culture; virus rna; cell transformation; cell nucleus; radioisotope; nucleic acid hybridization; clone cells; electrophoresis; cell transformation, viral; virus protein; chick embryo; clone; oncogene proteins, viral; quail; priority journal; oncovirinae; support, u.s. gov't, p.h.s.; leukosis virus, avian |
| Journal Title: | Virology |
| Volume: | 151 |
| Issue: | 1 |
| ISSN: | 0042-6822 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1986-05-01 |
| Start Page: | 131 |
| End Page: | 138 |
| Language: | English |
| DOI: | 10.1016/0042-6822(86)90111-x |
| PUBMED: | 3008428 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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