Specificity analysis of human monoclonal antibodies reactive with cell surface and intracellular antigens Journal Article

   


Authors: Cote, R. J.; Morrissey, D. M.; Houghton, A. N.; Thomson, T. M.; Daly, M. E.; Oettgen, H. F.; Old, L. J.
Article Title: Specificity analysis of human monoclonal antibodies reactive with cell surface and intracellular antigens
Abstract: Over 4350 human Ig-secreting hybrids have been generated through the fusion of human lymphocytes with NS-1 (mouse), LICR-2, SKO-007, GM4672, or UC729-6 (human) myeloma and lymphoblastoid cell lines. NS-1 proved to be the most satisfactory fusion partner, and 83% of the stable Ig-secreting clones were derived from NS-1 fusions. Three hundred five hybrids produced human monoclonal antibodies (hmAb) reactive with cell surface or intracellular antigens expressed by cultured human tumor cell lines, and 111 of these have undergone detailed serological specificity anaylsis. Several general points have emerged from our study of hmAb: (i) A significant proportion of the human B-cell clones produce antibody reactive with cellular antigens. (ii) The majority of these antigens have an intracellular location and are broadly distributed. (iii) Intracellular and cell surface differentiation antigens and other antigens with restricted distribution have been defined by hmAb, including two cell surface antigens not detected on normal cells. (iv) The relationship of these findings to cancer is unclear, as hmAb reactive with antigens showing distinctive distribution have been generated from the lymphocytes of normal individuals as well as tumor-bearing patients.
Keywords: immunofluorescence; in vitro study; tumor antigen; monoclonal antibody; membrane antigen; tumor cell line; intermediate filament; human; priority journal; blood and hemopoietic system
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 83
Issue: 9
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1986-05-01
Start Page: 2959
End Page: 2963
Language: English
DOI: 10.1073/pnas.83.9.2959
PROVIDER: scopus
PMCID: PMC323426
PUBMED: 3458255
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022476776&doi=10.1073%2fpnas.83.9.2959&partnerID=40&md5=5dd00607a4c8c915269c90097c1c6563
Notes: Article -- Export Date: 18 August 2021 -- Source: Scopus
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MSK Authors
  1. Herbert F Oettgen
    130 Oettgen
  2. Alan N Houghton
    364 Houghton
  3. Lloyd J Old
    593 Old
  4. Richard J. Cote
    27 Cote
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