Cell-surface antigens of melanoma recognized by human monoclonal antibodies Journal Article
| Authors: | Yamaguchi, H.; Furukawa, K.; Fortunato, S. R.; Livingston, P. O.; Lloyd, K. O.; Oettgen, H. F.; Old, L. J. |
| Article Title: | Cell-surface antigens of melanoma recognized by human monoclonal antibodies |
| Abstract: | Human monoclonal antibodies (mAbs) were derived from lymph node lymphocytes and peripheral blood lymphocytes (PBL) from patients with melanoma. Four methods for generating human mAbs were compared: fusion with human [LICR-LON-HMy-2 (LICR-2)] or mouse (NS-1) cells; transformation by Epstein-Barr virus (EBV); and EBV transformation followed by NS-1 fusion. NS-1 fusion with lymph node lymphocytes resulted in a higher number of growing hybrids than LICR-2 fusion. Virtually no hybrids were obtained from NS-1 or LICR-2 fusions with PBL. EBV transformed lymphocytes from lymph node and peripheral blood with equal efficiency, and the yield of proliferating cultures for antibody screening was more than 10- to 30-fold greater than that obtained by fusion techniques. However, once antibody-producing cultures had been identified, stability and clonability of EBV-transformed cells were poorer than that of NS-1 hybrid cells. To combine the strengths of both methods, cultures of EBV-transformed cells were fused with NS-1, and hybrid clones were isolated that showed vigorous growth, clonability, and stable antibody secretion. Detailed specificity analysis of the mAbs produced by six of these clones indicated detection of a class 1 (unique) melanoma antigen, a class 3 melanoma antigen, and four ganglioside antigens (GD3, GM3, and two other, as yet uncharacterized, heterophile antigens). |
| Keywords: | human cell; melanoma; cell line; in vitro study; tumor antigen; monoclonal antibody; lymphatic system; blood; antibodies, monoclonal; cell culture; lymph node; diagnosis; lymphocytes; clone cells; cell transformation, viral; herpesvirus 4, human; antigens, surface; antigen-antibody complex; humans; human; priority journal; blood and hemopoietic system |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 84 |
| Issue: | 8 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1987-04-01 |
| Start Page: | 2416 |
| End Page: | 2420 |
| Language: | English |
| DOI: | 10.1073/pnas.84.8.2416 |
| PUBMED: | 3031684 |
| PROVIDER: | scopus |
| PMCID: | PMC304662 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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