A high-affinity monoclonal antibody (GIF-1) to human gamma-interferon: Neutralization of interferon mediated inhibition of retrovirus production and 2 ́-5 ́ (A) synthetase induction Journal Article
| Authors: | Feit, C.; Sen, G.; Bartal, A. H.; Hirshaut, Y.; Anderson, S. L.; Millet, S. K.; Rubin, B. Y. |
| Article Title: | A high-affinity monoclonal antibody (GIF-1) to human gamma-interferon: Neutralization of interferon mediated inhibition of retrovirus production and 2 ́-5 ́ (A) synthetase induction |
| Abstract: | An hybridoma clone secreting an IgG1 monoclonal antibody (GIF-1) specific for human gamma-interferon (HuIFN-γ) has been generated using HAT medium supplemented with insulin (HIAT) at the initial stage of cell fusion. This antibody is capable of neutralizing the antiviral activity of HuIFN-γ, the ability of HuIFN-γ to inhibit retroviral replication in RD-114 cells, and the ability of HuIFN-γ to induce the 2 ́-5 ́ oligoadenylate (A) synthetase in RD-114 and HeLa cells. Eluate from an immunoaffinity column containing GIF-1 yielded two protein bands of molecular weight of 20 and 25 kd when subjected to SDS-PAGE. © 1986 S. Karger AG, Basel. |
| Keywords: | drug efficacy; nonhuman; animals; mice; in vitro study; mice, inbred balb c; monoclonal antibody; antibodies, monoclonal; gamma interferon; cell culture; virus replication; molecular weight; electrophoresis; retrovirus; retroviridae; drug interaction; interferon type i; retroviruses; interferon type ii; neutralization tests; unidentified retrovirus; interferons; female; priority journal; vesicular stomatitis-indiana virus; 2',5'-oligoadenylate synthetase; 2 ́-5 ́ (a) synthetase |
| Journal Title: | Experimental Cell Biology |
| Volume: | 54 |
| Issue: | 4 |
| ISSN: | 0304-3568 |
| Publisher: | S. Karger AG |
| Date Published: | 1986-01-01 |
| Start Page: | 212 |
| End Page: | 219 |
| Language: | English |
| DOI: | 10.1159/000163357 |
| PUBMED: | 3023159 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
