Prostaglandin E counteracts the gamma interferon induction of major histocompatibility complex class-II antigens on U937 cells and induction of responsiveness of U937 colony-forming cells to suppression by lactoferrin, transferrin, acidic isoferritins, and prostaglandin E Journal Article
| Authors: | Piacibello, W.; Rubin, B. Y.; Broxmeyer, H. E. |
| Article Title: | Prostaglandin E counteracts the gamma interferon induction of major histocompatibility complex class-II antigens on U937 cells and induction of responsiveness of U937 colony-forming cells to suppression by lactoferrin, transferrin, acidic isoferritins, and prostaglandin E |
| Abstract: | The established human monoblast or early monocyte cell line, U937, was evaluated for modulating influences of prostaglandin E2 (PGE2) on human gamma interferon (HuIFN(γ)) induction of MHC class-II (Ia) antigens on U937 cells and the HuIFN(γ) induction of responsiveness of U937 colony-forming cells (CFC) to inhibition by lactoferrin (LF), transferrin (TF), acidic isoferritins (AIF), and prostaglandin E (PGE). U937 CFC were induced to a state of responsiveness to the suppressive influences of PGE by HuIFN(γ). When MHC class-II antigens were induced on U937 cells and the cells sorted on the fluorescence activated cell sorter (FACS) IV into positive and negative cells, colony formation by the MHC class-II antigen+ population of cells was suppressed by LF, TF, AIF, and PGE2. Colony formation by the sorted population of MHC class-II antigen- cells was not influenced significantly by LF, TF, AIF, or PGE2. When PGE was present in the suspension culture for 72 h with U937 cells exposed to HuIFN(γ) plus indomethacin, it blocked the induction of MHC class-II antigens as well as the associated inhibition of U937 CFC by LF, TF, AIF, and PGE2. |
| Keywords: | leukemia; human cell; drug efficacy; drug potentiation; flow cytometry; cell line; gamma interferon; drug mechanism; prostaglandin e2; hematopoietic stem cells; hematopoiesis; monocytes; histocompatibility antigens class ii; major histocompatibility complex; prostaglandin e; prostaglandins e; cell separation; ferritin; transferrin; dinoprostone; colony formation; lactoferrin; drug comparison; interferon type ii; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; lactoglobulins; preliminary communication; blood and hemopoietic system; isoferritin |
| Journal Title: | Experimental Hematology |
| Volume: | 14 |
| Issue: | 1 |
| ISSN: | 0301-472X |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1986-01-01 |
| Start Page: | 44 |
| End Page: | 50 |
| Language: | English |
| PUBMED: | 3080325 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus; Acknowledgments: We wish to thank Maria Wachter for running the samples on the FACS IV and Mrs. Cindy Booth for typing the manuscript. |