Major reorganization of immunoglobulin VH segmental elements during vertebrate evolution Journal Article
| Authors: | Hinds, K. R.; Litman, G. W. |
| Article Title: | Major reorganization of immunoglobulin VH segmental elements during vertebrate evolution |
| Abstract: | In mammals, the immunoglobulin heavy-chain variable region (VH) locus is organized in a linear fashion; individual VH, diversity (DH), joining (JH) and constant (CH) region segments are linked in separate regions1. During somatic development, coding segments flanked by characteristic short recombination signal sequences, separated by intervening sequence regions that may exceed 2,000 kilobases (kb), are recombined. Combinatorial joining of different segments as well as imprecision in this process contribute to the diversity of the primary antibody response; subsequent mutation further alters functionally rearranged genes. This basic somatic reorganization mechanism is shared by six major families of genes encoding antigen receptors2. Previously, we have shown that multiple germline genes and mammalian-like recombination signal sequences are associated with the VH gene family of Heterodontus francisci (horned shark), a primitive elasmobranch3. Studies presented here demonstrate that segmental reorganization involving mammalian-like DH and J H segments occurs in the lymphoid tissues of this species. In marked contrast to the mammalian system, we find multiple instances of close linkage (∼10 kb) between individual VH, DH, JH and CH segments. This unique organization may limit combinatorial joining and be a factor in the restricted antibody response of this lower vertebrate4,5. © 1986 Nature Publishing Group. |
| Keywords: | nonhuman; protein conformation; animal cell; animal; mice; heredity; biological model; cell line; evolution; lymphatic system; immunoglobulin gene; immunoglobulin variable region; amino acid sequence; genetic engineering; nucleotide sequence; base sequence; radioisotope; fish; antibody formation; normal value; human; priority journal; support, u.s. gov't, p.h.s.; immunoglobulins, heavy-chain |
| Journal Title: | Nature |
| Volume: | 320 |
| Issue: | 6062 |
| ISSN: | 0028-0836 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1986-04-10 |
| Start Page: | 546 |
| End Page: | 549 |
| Language: | English |
| DOI: | 10.1038/320546a0 |
| PUBMED: | 3083268 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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