Disulfide linkages between antigen-binding receptors on chicken B-lymphocytes Journal Article
| Authors: | Rosenspire, A. J.; Myung Soo, Lee; Pollak, S. V.; Yong Sung, Choi |
| Article Title: | Disulfide linkages between antigen-binding receptors on chicken B-lymphocytes |
| Abstract: | Membrane immunoglobulin receptors on chicken B-cells have been shown to display a heterogeneity with respect to interchain disulfide linkages. One fraction of the surface Ig (sIg) appears to display the traditional H2-L2 linkage. We also present evidence that this Ig is covalently bound via a disulfide linkage to actin. In this instance, the isolated Ig heavy chain, after reduction, has a mol. wt of 80 K. Perhaps more significantly, we show that another fraction of the sIg exists in a highly aggregated form that is stabilized by disulfide linkages. In contrast to the sIg found in the H2-L2 configuration, there is no evidence of actin within the aggregates and the sIg heavy chains isolated from these aggregates display a slightly faster mobility on SDS-PAGE under reducing conditions, running at about 77 K. Furthermore, it appears that the Ig within the large aggregates may have a higher avidity with respect to antigen binding, and so this Ig structure may be the more relevant to antigen-induced receptor-mediated signaling in the B-cell. © 1986. |
| Keywords: | nonhuman; animal; actin; spleen; b lymphocyte; b-lymphocytes; lymphatic system; molecular weight; disulfide; disulfides; electrophoresis, polyacrylamide gel; chickens; receptors, antigen, b-cell; immunoglobulin receptor; chicken; chromatography, affinity; priority journal; support, u.s. gov't, p.h.s.; immunoglobulins, heavy-chain; immunoglobulins, surface |
| Journal Title: | Molecular Immunology |
| Volume: | 23 |
| Issue: | 1 |
| ISSN: | 0161-5890 |
| Publisher: | Pergamon-Elsevier Science Ltd |
| Date Published: | 1986-01-01 |
| Start Page: | 1 |
| End Page: | 13 |
| Language: | English |
| DOI: | 10.1016/0161-5890(86)90166-5 |
| PUBMED: | 3083239 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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