Glucose metabolism in human gliomas: Correspondence of in situ and in vitro metabolic rates and altered energy metabolism Journal Article
| Authors: | Galarraga, J.; Loreck, D. J.; Graham, J. F.; DeLaPaz, R. L.; Smith, B. H.; Hallgren, D.; Cummins, C. J. |
| Article Title: | Glucose metabolism in human gliomas: Correspondence of in situ and in vitro metabolic rates and altered energy metabolism |
| Abstract: | The rates of disappearance of glucose from the medium of 13 human glioma-derived cell lines and one cultured of normal human cortical astrocytes were determined by ftuorometric techniques. High-grade glioma-derived cultures showed a range of glucose consumption between 1 and 5 nmol/min/mg protein. Normal astrocyte cultures and cultures derived from grades I-III gliomas had a glucose consumption rate of 2-3 nmol/min/mg protein. Seven high-grade glioma lines were derived from surgical samples taken from patients who had been scanned by18F-2-deoxy-d-glucose positron computed tomography. The rate of glucose consumption in these high-grade glioma-derived lines was close to the maximum local cerebral metabolic rate for glucose (LCMRglc) measured in situ in the tumors from which the cultures were derived. In cultured glioma-derived lines, approximately one-half of the glucose consumed was recovered as lactate and pyruvate, suggesting a reliance of glioma cells on aerobic glycolysis. ATP and phosphocreatine (PCr) levels were variable in the gliomaderived lines, and ATP was lower in the glioma-derived lines than in the normal astrocytes. Levels and regulation of glycogen differed significantly among the various glioma-derived cell lines. Glycogen content did not diminish as glucose was consumed, suggesting that glycogen utilization is not tightly regulated by the glucose metabolic rate. These results suggest that human glioma-derived cell cultures (1) adequately reflect the metabolic capacity of gliomas in situ and (2) are significantly altered in several aspects of their glycolytic metabolism. © 1986 Plenum Publishing Corporation. |
| Keywords: | glioma; metabolism; tumor cells, cultured; cell culture; glucose; adenosine triphosphate; energy metabolism; pyruvic acid derivative; glia; pyruvic acid; creatine phosphate; gliomas; phosphocreatine; human; article; pyruvates; positron emission computed tomography |
| Journal Title: | Metabolic Brain Disease |
| Volume: | 1 |
| Issue: | 4 |
| ISSN: | 0885-7490 |
| Publisher: | Plenum Press |
| Date Published: | 1986-12-01 |
| Start Page: | 279 |
| End Page: | 291 |
| Language: | English |
| DOI: | 10.1007/bf00999357 |
| PUBMED: | 3508247 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work