Detection of RNA transcripts in normal lymphoid and myeloid colonies Journal Article
| Authors: | Paige, C. J.; Wu, G. E.; Castiglia, C. L. |
| Article Title: | Detection of RNA transcripts in normal lymphoid and myeloid colonies |
| Abstract: | A procedure is described for the routine detection of RNA transcripts in small numbers of hematopoietic cells growing in semi-solid agar. It is suggested that hybridization depends upon RNA expression and that as few as 2500 mRNA molecules per colony are easily detected. Applications of this technique are described in three diverse experimental systems; immunoglobulin gene expression in B cell colonies; neo expression in normal and transformed B cell clones derived from multipotent stem cells infected with a neo-containing retrovirus; and c-myc expression in factor-dependent myeloid colonies. © 1986. |
| Keywords: | cells, cultured; heredity; bone marrow; immunoglobulin; transcription, genetic; in vitro study; b lymphocyte; b-lymphocytes; stem cell; rna; immunoglobulin gene; genetic engineering; hematopoietic cell; hematopoietic stem cells; nucleic acid hybridization; c-myc; retrovirus; immunoglobulins; clone; proto-oncogenes; dna transposable elements; genes, bacterial; human; priority journal; support, non-u.s. gov't; neo; blood and hemopoietic system; hybridization, in situ |
| Journal Title: | Journal of Immunological Methods |
| Volume: | 93 |
| Issue: | 1 |
| ISSN: | 0022-1759 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1986-10-23 |
| Start Page: | 37 |
| End Page: | 43 |
| Language: | English |
| DOI: | 10.1016/0022-1759(86)90430-8 |
| PUBMED: | 2430020 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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