Constitutive c-myc expression enhances the response of murine mast cells to IL-3, but does not eliminate their requirement for growth factors Journal Article
| Authors: | Hume, C. R.; Nocka, K. H.; Sorrentino, V.; Lee, J. S.; Fleissner, E. |
| Article Title: | Constitutive c-myc expression enhances the response of murine mast cells to IL-3, but does not eliminate their requirement for growth factors |
| Abstract: | An interleukin-3 (IL-3) dependent mast cell line (MC) was infected with a recombinant retrovirus expressing the proto-oncogene c-myc and the drug selectable marker neo. Cells containing the transcriptionally activated c-myc gene displayed an increased growth rate in liquid culture and a higher cloning efficiency in soft agar when compared to control virus infected cells. All infected cells remained absolutely dependent on IL-3 for growth and were not tumorigenic in nude mice. Similar results were obtained with two additional IL-3 dependent cell lines, the mast cell 32D and the pre-B-cell Ea3. Thus, while constitutive expression of c-myc potentiates the response of mast cells to IL-3, it is not sufficient to eliminate their requirement for growth factors. |
| Keywords: | proto-oncogene proteins; nonhuman; animal cell; mouse; animal; mice; cell division; gene expression; gene expression regulation; cell culture; rna, messenger; oncogene c myc; retrovirus; growth substances; mast cell; mast cells; interleukin 3; interleukin-3 |
| Journal Title: | Oncogene |
| Volume: | 2 |
| Issue: | 3 |
| ISSN: | 0950-9232 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1988-01-01 |
| Start Page: | 223 |
| End Page: | 226 |
| Language: | English |
| PUBMED: | 3281092 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
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