Reconstruction of adenovirus replication origins with a human nuclear factor I binding site Journal Article
| Authors: | Adhya, S.; Shneidman, P. S.; Hurwitz, J. |
| Article Title: | Reconstruction of adenovirus replication origins with a human nuclear factor I binding site |
| Abstract: | Nuclear factor I is a host-coded DNA-binding protein that stimulates initiation of adenovirus DNA replication. To understand the mechanism of action of nuclear factor I, we have constructed, by recombinant DNA techniques, origins of replication in which the adenovirus type 5 nuclear factor I binding site (FIB site) has been replaced by a FIB site isolated from human genomic DNA (Gronostajski, R.M., Nagata, K., and Hurwitz, J. (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 4013-4017). Assays of such recombinants for initiation and elongation in vitro showed that nuclear factor I was active only when the FIB site was relatively close to the DNA terminus, i.e. the FIB site was centered at nucleotides 30-36 from the end of the DNA. Nuclear factor I was active in either orientiation within this distance range. The presence of one or two additional FIB sites in the downstream region had no effect. The implications of these results for the mechanism of nuclear factor I action are discussed. |
| Keywords: | dna binding protein; dna-binding proteins; nonhuman; dna replication; molecular cloning; genetic engineering; plasmid; base sequence; dna, viral; binding sites; radioisotope; virus replication; nucleic acid hybridization; nucleic acid conformation; adenoviridae; adenovirus; deoxyribonuclease i; human; priority journal; dna restriction enzymes; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 261 |
| Issue: | 7 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1986-03-05 |
| Start Page: | 3339 |
| End Page: | 3346 |
| Language: | English |
| PUBMED: | 3005289 |
| PROVIDER: | scopus |
| DOI: | 10.1016/S0021-9258(17)35788-5 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
