A new supertypic HLA class II determinant (PL2) differentially expressed with DR7, DRw11(5), DRw13(w6), DRw14(w6), DR3, and DR2 and biochemically localized to DR7 molecules Journal Article
| Authors: | Horibe, K.; Knowles, R. W.; Dupont, B. |
| Article Title: | A new supertypic HLA class II determinant (PL2) differentially expressed with DR7, DRw11(5), DRw13(w6), DRw14(w6), DR3, and DR2 and biochemically localized to DR7 molecules |
| Abstract: | A monoclonal antibody, PL2, has been produced that reacts with a new supertypic determinant expressed on the peripheral blood B lymphocytes and B-leukemic cells (B-CLL) from all individuals who are HLA-DR7 and some individuals who are HLA-DR5 positive. The genetic linkage of the PL2 determinant to the HLA region was demonstrated by family segregation studies. When cultured Epstein-Barr virus (EBV) transformed B cell lines were examined, PL2 was again found to be expressed on all cell lines homozygous for HLA-DR7 and the DRw11(5) subtype of HLA-DR5 positive cells, while one DRw12(5) cell line was negative, suggesting PL2 may distinguish between these DR5 subtypes. In addition, using the panel of EBV-transformed B-cell lines, PL2 was also found to be weakly expressed on HLA-DRw14(w6), -DRw13(w6), -DR3, and -DR2 positive cells but was completely absent from HLA-DR1 and -DR4 positive cells, and is probably absent also from DRw8- and DRw10-positive cells. From titration analysis and quantitative absorption studies the PL2 determinant was found to be expressed at quantitatively different levels in the following order: DR7> DRw11, DRw14>DRw13>Dr3>DR2. The molecules carrying the PL2 determinant on DR7 cells have been characterized biochemically to be a subpopulation of HLA class II molecules recognized by the DR specific monoclonal antibody, L243. Furthermore, by two-dimensional gel analysis, PL2 immunoprecipitated only two of three beta chains associated with the DR-alpha chain, which are the same two chains that carry the DR7 allodeterminants. Thus, PL2 was found to recognized a new supertypic determinant, which on DR7 cell lines is expressed on a subpopulation of HLA-DR molecules. © 1986. |
| Keywords: | animal; mice; cell line; b lymphocyte; b-lymphocytes; monoclonal antibody; mice, inbred strains; hla dr antigen; hla-dr antigens; cytotoxicity, immunologic; histocompatibility antigens class ii; epitopes; leukemia, lymphocytic; hla-dr5 antigen; hla-dr3 antigen; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; hla-dr7 antigen; hla-dr2 antigen; blood and hemopoietic system; hla-dr6 antigen |
| Journal Title: | Human Immunology |
| Volume: | 16 |
| Issue: | 1 |
| ISSN: | 0198-8859 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1986-05-01 |
| Start Page: | 14 |
| End Page: | 23 |
| Language: | English |
| DOI: | 10.1016/0198-8859(86)90032-7 |
| PUBMED: | 2423484 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus; Acknowledgments: We thank Dr. Fritz H. Bach for providing MoAbs and Ms. Bernadette Kienzle for her excellent technical assistance. |
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