Mapping of a restriction fragment length polymorphism associated with defective DR β4 chain expression to the HLA-DRB1 gene Journal Article
| Authors: | Sutton, V. R.; Knowles, R. W. |
| Article Title: | Mapping of a restriction fragment length polymorphism associated with defective DR β4 chain expression to the HLA-DRB1 gene |
| Abstract: | The HLA-DR β4 chaine, encoded by the DRB4 gene, carries two DRw53 determinants normally expressed by DR4, DR7, and DR9 individuals. However, some DR7 individuals (DR7,Dw11) fail to express the DR β4 chain. At the genomic level, a HindIII restriction fragment length polymorphism can be detected in these individuals with a DRβ cDNA probe. The association of this altered HindIII fragment with defective β4 chain expression suggested the possibility that the polymorphic fragment was derived from the DRB4 gene and might, therefore, be related to the defect in expression. However, detailed Southern blot analysis has now mapped the polymorpbic fragment to the 3′ end of the DRB1 gene, approximately 100 kb away from the defective DRB4 gene. Although the alteration in the DRB1 gene might involve sequences important in regulating the expression of the DRB4 gene, it is more likely that the association results from strong positive linkage disequilibrium between these DR β chain genes. © 1988. |
| Keywords: | human cell; exons; biological model; cell line; monoclonal antibody; gene expression regulation; cell culture; hla dr antigen; hla-dr antigens; gene control; hla-d antigens; chromosome mapping; plasmid dna; southern blotting; restriction fragment length polymorphism; polymorphism, restriction fragment length; human; male; female; priority journal; dna restriction enzymes; polymorphism (genetics); support, non-u.s. gov't; support, u.s. gov't, p.h.s.; deoxyribonuclease hindiii; hla-dr4 antigen; dna mapping |
| Journal Title: | Human Immunology |
| Volume: | 22 |
| Issue: | 2 |
| ISSN: | 0198-8859 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1988-06-01 |
| Start Page: | 123 |
| End Page: | 134 |
| Language: | English |
| DOI: | 10.1016/0198-8859(88)90042-0 |
| PUBMED: | 2901408 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
