Structural alterations in the 5′ region of the BCL2 gene in follicular lymphomas with BCL2‐MBR or BCL2‐MCR rearrangements Journal Article
| Authors: | Mikraki, V.; Ladanyi, M.; Chaganti, R. S. K. |
| Article Title: | Structural alterations in the 5′ region of the BCL2 gene in follicular lymphomas with BCL2‐MBR or BCL2‐MCR rearrangements |
| Abstract: | We report a new class of molecular lesions in the 5′ region of the BCL2 protooncogene when it undergoes a t(14;18) translocation‐associated rearrangement in the major break cluster (MBR) or minor break cluster (MCR) regions. Among 52 tumors assayed for BCL2 rearrangements using the MBR, MCR, and 5′ probes, seven (six with MBR and one with MCR translocation breaks) showed aberrant bands in unique enzyme digests of DNA hybridized with the 5′ probe. The aberrant bands in four tumors were in HindIII digests, in two they were in EcoRI digests, while the aberrant band in one was in a BamHI digest. The two EcoRI bands and two of the four HindIII bands were of identical sizes. Germline polymorphisms as the source of these bands was ruled out by appropriate control experiments. These results suggest that the bands were derived from point mutations or small deletions in the 5′ region of BCL2. The significance of this alteration to BCL2 function in translocation‐carrying tumors remains to be determined. Copyright © 1991 Wiley‐Liss, Inc., A Wiley Company |
| Keywords: | human tissue; gene deletion; proto-oncogene proteins; comparative study; tumor markers, biological; dna, neoplasm; lymphoma; chromosome translocation; dna mutational analysis; point mutation; chromosome deletion; proto-oncogene proteins c-bcl-2; lymphoma, follicular; genetic markers; gene structure; proto-oncogenes; restriction mapping; chromosomes, human, pair 14; genes, immunoglobulin; chromosomes, human, pair 18; human; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; dna probe; translocation (genetics); immunoglobulins, heavy-chain |
| Journal Title: | Genes Chromosomes and Cancer |
| Volume: | 3 |
| Issue: | 2 |
| ISSN: | 1045-2257 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 1991-03-01 |
| Start Page: | 117 |
| End Page: | 121 |
| Language: | English |
| DOI: | 10.1002/gcc.2870030206 |
| PUBMED: | 1906343 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 September 2019 -- Source: Scopus |
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