Hormonal regulation of thyrotropin alpha and beta subunit mRNAs Journal Article
| Authors: | Gurr, J. A.; Vrontakis, M. E.; Athanasian, E. A.; Wagner, C. R.; Kourides, I. A. |
| Article Title: | Hormonal regulation of thyrotropin alpha and beta subunit mRNAs |
| Abstract: | We have examined the effects of 3,5,3'-triiodo-L-thyronine (T3), dexamethasone, bromocriptine, thyrotropin releasing hormone (TRH) and estrogen on the levels of pituitary α and TSH-β protein and mRNA levels in hypothyroid mice. After 3 days of treatment with T3 (0.5 μg/100 g body weight) serum TSH, α and TSH-β levels were 77%, 79% and 44% of control, respectively. Pituitary α and TSH-β-mRNA content was estimated by dot blot hybridization of total RNA with 32P-labeled α and TSH-β plasmid probes. There was no change in α mRNA after 3 days of T3 treatment but TSH-β mRNA had decreased to 60% of control. With T3 at 2 μg/100 g body weight for 3 days, TSH protein was 27% of control and TSH-β was undetectable, but there was no change in α. TSH-β-mRNA was decreased to 40% of control at 1 day and was barely detectable at 3 days, whereas α mRNA was 70% of control at 1 day and 42% at 3 days. Dexamethasone and bromocriptine caused no consistent change in pituitary levels of α and TSH-β-mRNA. Treatment with TRH caused small increases in serum TSH and both α and TSH-β-mRNA levels. Estrogen treatment increased serum TSH and subunit levels and TSH-β-mRNA, but not α. We conclude that thyroid hormones decrease α and β subunit mRNA levels discordantly in both the hypothyroid pituitary and in thyrotropic tumors and that the suppressive effect of thyroid hormone is the major regulator of TSH. |
| Keywords: | unclassified drug; drug efficacy; nonhuman; mouse; animal experiment; estrogen; dexamethasone; animalia; iodine 131; messenger rna; radioisotope; liothyronine; thyroid hormone; endocrine system; bromocriptine; intraperitoneal drug administration; priority journal; article; protirelin; polyestradiol phosphate; thyrotropin alpha subunit; thyrotropin beta subunit |
| Journal Title: | Hormone and Metabolic Research |
| Volume: | 18 |
| Issue: | 6 |
| ISSN: | 0018-5043 |
| Publisher: | Georg Thieme Verlag Kg |
| Date Published: | 1986-06-01 |
| Start Page: | 382 |
| End Page: | 385 |
| Language: | English |
| DOI: | 10.1055/s-2007-1012322 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
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