Effect of section thickness on quality of flow cytometric DNA content determinations in paraffin‐embedded tissues Journal Article

  

Authors:	Stephenson, R. A.; Gay, H.; Fair, W. R.; Melamed, M. R.
Article Title:	Effect of section thickness on quality of flow cytometric DNA content determinations in paraffin‐embedded tissues
Abstract:	DNA content determinations were carried out by flow cytometry on nuclear suspensions prepared from the same paraffin embedded tissue block for each of eight surgically resected human carcinomas at section thicknesses of 5,10,20,30,40,50, and 100 μm. Flow cytometric DNA determinations were also obtained on fresh tissue specimens in four of the eight carcinomas. As section thickness decreased below 50 μm, there was a progressive increase in the histogram baseline noise at low DNA values and a decrease in the relative peak height of aneuploid DNA. The former was attributed to an increase of nuclear fragments in thinner sections, and the latter to the greater probability of transection of the larger aneuploid cells within a specimen. Both artifacts were minimized at section thickness of 50 μm or greater. Copyright © 1986 Wiley‐Liss, Inc.
Keywords:	nonhuman; flow cytometry; animal cell; lung neoplasms; breast neoplasms; histology; kidney neoplasms; animalia; dna; carcinoma; cell nucleus; bladder neoplasms; paraffin; staining and labeling; microtomy; human; support, u.s. gov't, p.h.s.; paraffin embedded; microtome
Journal Title:	Cytometry
Volume:	7
Issue:	1
ISSN:	0196-4763
Publisher:	Wiley Liss
Date Published:	1986-01-01
Start Page:	41
End Page:	44
Language:	English
DOI:	10.1002/cyto.990070106
PUBMED:	2419054
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022597397&doi=10.1002%2fcyto.990070106&partnerID=40&md5=a83ac08a8b0501352c78d7fc774f4e96
Notes:	Article -- Export Date: 18 August 2021 -- Source: Scopus; Acknowledgments: We are grateful to S. Carter for careful and competent tech- nical assistance, to A. Scorza for help in preparing the tissue sections, and to G. Watson for excellent secretarial assistance.

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