Rapid immunodiagnosis of cytomegalovirus pneumonia by bronchoalveolar lavage using human and murine monoclonal antibodies Journal Article

  


Authors: Emanuel, D.; Peppard, J.; Stover, D.; Gold, J.; Armstrong, D.; Hammerling, U.
Article Title: Rapid immunodiagnosis of cytomegalovirus pneumonia by bronchoalveolar lavage using human and murine monoclonal antibodies
Abstract: Bronchoalveolar lavage material from 54 immunocompromised patients with interstitial pneumonia was examined by immunofluorescence with cytomegalovirus-specific monoclonal antibodies. Twelve patients (22%) had cytomegalovirus detected in their lavaged cells, and 9 of these patients (17%) had proven cytomegalovirus pneumonitis. This assay detected all samples with cytomegalovirus when the virus was detected by established methods either at the time of lavage or after any other procedure in the subsequent 2 months; that is, it had a sensitivity of 100%. Cytomegalovirus could be detected within 3 hours of lavage, and a clear correlation was seen between the number of fluorescent cells and the presence of cytomegalovirus pneumonia. All 9 patients with pneumonitis had more than 0.5% fluorescent cells, whereas the 3 patients in whom cytomegalovirus was detected without pneumonia had significantly fewer fluorescent cells. This method provides a sensitive, rapid, and quantifiable system for detection of cytomegalovirus, facilitating the early diagnosis and treatment of cytomegalovirus pneumonia.
Keywords: clinical article; immunofluorescence; monoclonal antibody; diagnosis; serodiagnosis; acquired immune deficiency syndrome; drug therapy; immunosuppressive treatment; bone marrow transplantation; therapy; cytomegalovirus; intravenous drug administration; interstitial pneumonia; lung lavage; respiratory system; virus characterization; human; priority journal; blood and hemopoietic system
Journal Title: Annals of Internal Medicine
Volume: 104
Issue: 4
ISSN: 0003-4819
Publisher: American College of Physicians  
Date Published: 1986-04-01
Start Page: 476
End Page: 480
Language: English
DOI: 10.7326/0003-4819-104-4-476
PROVIDER: scopus
PUBMED: 3006566
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022634453&doi=10.7326%2f0003-4819-104-4-476&partnerID=40&md5=aac7ce36ceda6f33794e0b26d985d15f
Notes: Article -- Export Date: 18 August 2021 -- Source: Scopus; Acknowledgments: The authors thank T. Pumerola MD, S. Kozinski MD, C. Cardon-Cardo MD, and Phillipa Rosalle for their help with this study; and Centocor Inc, Malvern, PA for its support.
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MSK Authors
  1. Diane E Stover-Pepe
    109 Stover-Pepe
  2. Ulrich Hammerling
    79 Hammerling
  3. Donald Armstrong
    240 Armstrong
  4. Jonathan W. M. Gold
    73 Gold
  5. David J. Emanuel
    28 Emanuel
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