The use of insulin-enriched culture medium and human-human hybridoma formation: A preliminary study Journal Article
| Authors: | Bartal, A. H.; Feit, C.; Hirshaut, Y. |
| Article Title: | The use of insulin-enriched culture medium and human-human hybridoma formation: A preliminary study |
| Abstract: | Human lymphoblastoid cell line WI-L2-729-HFZ was fused with human lymph-node lymphocytes in one fusion and with human spleen cells in another fusion to generate human-human hybridomas. In both, increasing doses of insulin were added to the HAT medium immediately after the PEG-mediated cell fusion (10-1-10-5 IU/ml) and the number of clones formed was determined 3 weeks later. 10-3 IU/ml of insulin resulted in a 2- to 5-fold increase in the number of clones generated compared to the control plates. In view of the known difficulties in generating high-yield human-human hybridoma fusions, it is suggested that the use of insulin-supplemented HAT medium may provide a more efficient way in obtaining such clones. |
| Keywords: | human cell; spleen; culture medium; in vitro study; lymphatic system; insulin; lymphocyte; lymphoblastoid cell line; spleen cell; hybrid cell; human; priority journal; blood and hemopoietic system |
| Journal Title: | Acta Haematologica |
| Volume: | 76 |
| Issue: | 1 |
| ISSN: | 0001-5792 |
| Publisher: | S. Karger AG |
| Date Published: | 1986-01-01 |
| Start Page: | 50 |
| End Page: | 53 |
| Language: | English |
| DOI: | 10.1159/000206018 |
| PUBMED: | 3098027 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work