Aberrant methylation in pediatric myelodysplastic syndrome Journal Article
| Authors: | Vidal, D. O.; Paixão, V. A.; Brait, M.; Souto, E. X.; Caballero, O. L.; Lopes, L. F.; Vettore, A. L. |
| Article Title: | Aberrant methylation in pediatric myelodysplastic syndrome |
| Abstract: | Background: Aberrant methylation of gene promoter region is responsible for inappropriate gene silencing, and it has been associated to initiation and progression of cancer. Aberrant promoter methylation is frequently observed in adult patients with myelodysplastic syndrome (MDS), but in pediatric patients it has been poorly investigated. Methods: We examined the promoter methylation status of 13 genes in bone marrow cells collected at diagnosis of 21 pediatric patients with MDS (subtype RAEB or RAEB-t). For this analysis, we performed sodium bisulfite treatment of genomic DNA, followed by methylation specific PCR (MSP). Results: In pediatric MDS samples, we observed two genes frequently methylated: CALCA was methylated in 85.7% (18/21) of the analyzed samples and CDKN2B in 50% (6/12). Conclusions: Our findings indicate that CALCA and CDKN2B are frequently methylated in pediatric MDS. It suggests that aberrant methylation in pediatric MDS seems to be similar to adult MDS, thus pediatric patients could be also benefited with treatment using demethylating agents. © 2006 Elsevier Ltd. All rights reserved. |
| Keywords: | adolescent; child; clinical article; controlled study; child, preschool; human cell; methylation; promoter region; cancer patient; genetic analysis; polymerase chain reaction; gene expression profiling; gene frequency; gene product; calcitonin; uvomorulin; dna methylation; myelodysplastic syndrome; dna; infant; gene identification; leukemogenesis; bone marrow cell; methylated dna protein cysteine methyltransferase; chromosome aberrations; apc protein; genomic dna; myelodysplastic syndromes; clinical observation; tissue inhibitor of metalloproteinase 3; sample; sequence analysis, dna; bisulfite; protein precursors; demethylation; cyclin dependent kinase 2; cyclin dependent kinase 2a; cyclin-dependent kinase inhibitor p15; protein p73; childhood disease; protein hic1; refractory anemia with excess blasts; protein p14arf; death associated protein kinase; msp; pediatric patients; cyclin dependent kinase 2b; protein calca; protein cdh13; protein rar beta; refractory anemia with excess blasts in transformation |
| Journal Title: | Leukemia Research |
| Volume: | 31 |
| Issue: | 2 |
| ISSN: | 0145-2126 |
| Publisher: | Elsevier Ltd |
| Date Published: | 2007-02-01 |
| Start Page: | 175 |
| End Page: | 181 |
| Language: | English |
| DOI: | 10.1016/j.leukres.2006.06.013 |
| PUBMED: | 16890288 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 9" - "Export Date: 17 November 2011" - "CODEN: LERED" - "Source: Scopus" |
