Cas9-cleavage sequences in size-reduced plasmids enhance nonviral genome targeting of CARs in primary human T cells Journal Article
| Authors: | Jing, R.; Jiao, P.; Chen, J.; Meng, X.; Wu, X.; Duan, Y.; Shang, K.; Qian, L.; Huang, Y.; Liu, J.; Huang, T.; Jin, J.; Chen, W.; Zeng, X.; Yin, W.; Gao, X.; Zhou, C.; Sadelain, M.; Sun, J. |
| Article Title: | Cas9-cleavage sequences in size-reduced plasmids enhance nonviral genome targeting of CARs in primary human T cells |
| Abstract: | T cell genome editing holds great promise to advance a range of immunotherapies but is encumbered by the dependence on difficult-to-produce and expensive viral vectors. Here, small double-stranded plasmid DNA modified to mediate high-efficiency homologous recombination is designed. The resulting chimeric antigen receptor (CAR)-T cells display a similar phenotype, transcriptional profile, and in vivo potency to CAR-T cells generated using adeno-associated viral vector. This method should simplify and accelerate the use of precision engineering to produce edited T cells for research and clinical purposes. © 2021 Wiley-VCH GmbH |
| Keywords: | cytology; homologous recombination; genes; clinical research; adoptive cell therapy; t-cells; antigen receptors; viral vectors; high-efficiency; cell engineering; car; crispr/cas9; precision engineering; genome targeting; nonviral methods; size-reduced plasmid; cell genome; double-stranded plasmid; nonviral; primary human t cells |
| Journal Title: | Small Methods |
| Volume: | 5 |
| Issue: | 7 |
| ISSN: | 2366-9608 |
| Publisher: | Wiley-VCH GmbH |
| Date Published: | 2021-07-15 |
| Start Page: | 2100071 |
| Language: | English |
| DOI: | 10.1002/smtd.202100071 |
| PROVIDER: | scopus |
| PUBMED: | 34927998 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 2 August 2021 -- Source: Scopus |
