Reconstitution of the human nuclear RNA exosome Journal Article

   


Authors: Januszyk, K.; Weick, E. M.; Lima, C. D.
Article Title: Reconstitution of the human nuclear RNA exosome
Abstract: We describe procedures to clone, express, and reconstitute an active human nuclear RNA exosome. Individual recombinant subunits are expressed from E. coli and successfully reconstituted into the nuclear complex, which contains the noncatalytic nine-subunit exosome core, the endoribonuclease and exoribonuclease DIS3, the distributive exoribonuclease EXOSC10, the cofactors C1D and MPP6, and the RNA helicase MTR4.
Keywords: ribonuclease; complex; core; subunit; decay; human; rna decay; rna exosome; paths; rrp47; exoribonuclease dis3l2; mpp6
Journal Title: Methods in Molecular Biology
Volume: 2062
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2020-01-01
Start Page: 467
End Page: 489
Language: English
ACCESSION: WOS:000651585300024
PROVIDER: wos
PUBMED: 31768991
DOI: 10.1007/978-1-4939-9822-7_23
PMCID: PMC8584190
Notes: Chapter 23 in "The Eukaryotic RNA Exosome" (ISBN: 978-1-4939-9822-7) -- Source: Wos
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MSK Authors
  1. Kurt Januszyk
    9 Januszyk
  2. Christopher D Lima
    103 Lima
  3. Eva-Maria Weick
    5 Weick
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