Oropharyngeal aspiration of ricin as a lung challenge model for evaluation of the therapeutic index of antibodies against ricin A-chain for post-exposure treatment Journal Article
| Authors: | Pratt, T. S.; Pincus, S. H.; Hale, M. L.; Moreira, A. L.; Roy, C. J.; Tchou-Wong, K. M. |
| Article Title: | Oropharyngeal aspiration of ricin as a lung challenge model for evaluation of the therapeutic index of antibodies against ricin A-chain for post-exposure treatment |
| Abstract: | To investigate the effectiveness of passive antibody treatment as post-exposure therapy for ricin, we had developed an oropharyngeal aspiration model for ricin lethal challenge and antibody administration. When polyclonal anti-deglycosylated ricin A-chain antibody (dgA Ab) was administered between 1-18 hr after ricin challenge, all animals survived while delayed treatment to 24 hr resulted in 30% survival. The protective effects of dgA Ab correlated with inhibition of apoptosis in the lungs in vivo and in RAW264.7 macrophage and Jurkat T cells in vitro. In addition, ricin-induced cell cytotoxicity was inhibited by both dgA Ab and RAC18 monoclonal antibody against ricin A-chain. Administration of RAC18 monoclonal antibody at 4, 18, and 24 hr after ricin exposure resulted in 100%, 60% and 50% protection, respectively, suggesting that the therapeutic window for passive vaccination extended to at least 24 hr post-ricin lung challenge. Copyright © Informa Healthcare USA, Inc. |
| Keywords: | survival; controlled study; human cell; nonhuman; animal cell; mouse; animals; mice; cell survival; apoptosis; lung toxicity; aspiration; cell line; inflammation; in vivo study; cytotoxicity; mice, inbred balb c; antibodies, monoclonal; evaluation; lung; antibodies; cell protection; lethality; oropharynx; experimental model; passive immunization; provocation test; lung aspiration; ricin; passive antibody; post-exposure treatment; ricin a; ricin antibody; pulmonary edema |
| Journal Title: | Experimental Lung Research |
| Volume: | 33 |
| Issue: | 8-9 |
| ISSN: | 0190-2148 |
| Publisher: | Informa Healthcare |
| Date Published: | 2007-10-01 |
| Start Page: | 459 |
| End Page: | 481 |
| Language: | English |
| DOI: | 10.1080/01902140701731805 |
| PUBMED: | 17994372 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 8" - "Export Date: 17 November 2011" - "CODEN: EXLRD" - "Source: Scopus" |
