T cell depletion of human bone marrow: Comparison of Campath-1 plus complement, anti-T cell ricin A chain immunotixin, and soybean agglutinin alone or in combination with sheep erythrocytes or immunomagnetic beads Journal Article

  

Authors:	Frame, J. N.; Collins, N. H.; Cartagena, T.; Waldmann, H.; O'Reilly, R. J.; Dupont, B.; Kernan, N. A.
Article Title:	T cell depletion of human bone marrow: Comparison of Campath-1 plus complement, anti-T cell ricin A chain immunotixin, and soybean agglutinin alone or in combination with sheep erythrocytes or immunomagnetic beads
Abstract:	The aim of this study was to compare the extent of in vitro T cell depletion and recovery of hematopoietic progenitor cells achieved with five methods of T cell depletion. Bone marrow samples from the same source were treated with monoclonal antibody Campath-1 (CP1) and human complement, XomaZyme-H65 (anti- T cell ricin A chain immunotoxin), or soybean agglutinin (SBA) alone or in combination with sheep erythrocytes (EAET) or a cocktail of immunomagnetic beads (B) directly coated with anti-CD2, anti-CD3, or anti-CD8 monoclonal antibodies. Residual T cells were enumerated by limiting dilution analysis, EAET rosetting, and proliferative responses to phytohemagglutinin. The results of this study demonstrated the following reductions in BM T cells as detected by limiting dilution analysis (mean % control): SBA+B (99.9%), SBA+EAET (99.8%), CP1+C' (99.4%), anti—T cell ricin A chain immunotoxin (99.0%), and SBA alone (94.2%). Neither PHA response nor enumeration of residual EAET rosettes provided discriminating differences in the degree of T cell depletion. © 1989 by The Williams and Wilkins Co.
Keywords:	human cell; comparative study; methodology; t lymphocyte; t-lymphocytes; animal; monoclonal antibody; lymphocyte activation; antibodies, monoclonal; leukocytes, mononuclear; hematopoietic stem cells; erythrocyte; rats; rosette formation; leukocyte count; drug combinations; bone marrow transplantation; colony-forming units assay; lymphocyte depletion; erythrocytes; depletion; complement; immunotoxins; microspheres; sheep; phytohemagglutinin; agglutinin; lectins; soybeans; human; priority journal; antitoxin; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title:	Transplantation
Volume:	47
Issue:	6
ISSN:	0041-1337
Publisher:	Lippincott Williams & Wilkins
Date Published:	1989-06-01
Start Page:	984
End Page:	988
Language:	English
PUBMED:	2660360
PROVIDER:	scopus
DOI:	10.1097/00007890-198906000-00013
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024362237&partnerID=40&md5=2648704a75b9027ad8ebc799f79991ed
Notes:	Article -- Export Date: 14 April 2020 -- Source: Scopus

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