In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates Journal Article
| Authors: | Ma, W.; Zheng, G.; Xie, W.; Mayr, C. |
| Article Title: | In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates |
| Abstract: | Liquid-like condensates have been thought to be sphere-like. Recently, various condensates with filamentous morphology have been observed in cells. One such condensate is the TIS granule network that shares a large surface area with the rough endoplasmic reticulum and is important for membrane protein trafficking. It has been unclear how condensates with mesh-like shapes but dynamic protein components are formed. In vitro and in vivo reconstitution experiments revealed that the minimal components are a multivalent RNA-binding protein that concentrates RNAs that are able to form extensive intermolecular mRNA–mRNA interactions. mRNAs with large unstructured regions have a high propensity to form a pervasive intermolecular interaction network that acts as condensate skeleton. The underlying RNA matrix prevents full fusion of spherical liquid-like condensates, thus driving the formation of irregularly shaped membraneless organelles. The resulting large surface area may promote interactions at the condensate surface and at the interface with other organelles. © Ma et al. |
| Journal Title: | eLife |
| Volume: | 10 |
| ISSN: | 2050-084X |
| Publisher: | eLife Sciences Publications Ltd. |
| Date Published: | 2021-03-02 |
| Start Page: | e64252 |
| Language: | English |
| DOI: | 10.7554/eLife.64252 |
| PUBMED: | 33650968 |
| PROVIDER: | scopus |
| PMCID: | PMC7968931 |
| DOI/URL: | |
| Notes: | Article -- MSK author Gang Zhen's last name is misspelled on the original publication -- Export Date: 3 May 2021 -- Source: Scopus |
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