Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids Journal Article
| Authors: | Bojmar, L.; Kim, H. S.; Tobias, G. C.; Pelissier Vatter, F. A.; Lucotti, S.; Gyan, K. E.; Kenific, C. M.; Wan, Z.; Kim, K. A.; Kim, D.; Hernandez, J.; Pascual, V.; Heaton, T. E.; La Quaglia, M. P.; Kelsen, D.; Trippett, T. M.; Jones, D. R.; Jarnagin, W. R.; Matei, I. R.; Zhang, H.; Hoshino, A.; Lyden, D. |
| Article Title: | Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids |
| Abstract: | We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling. For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020). © 2020 The Author(s) We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples necessitates robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling. © 2020 The Author(s) |
| Keywords: | cell culture; cell membrane; cell isolation; exosomes; isolation; exomeres; extracellular vesicles and particles |
| Journal Title: | STAR Protocols |
| Volume: | 2 |
| Issue: | 1 |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press |
| Date Published: | 2021-03-19 |
| Start Page: | 100225 |
| Language: | English |
| DOI: | 10.1016/j.xpro.2020.100225 |
| PROVIDER: | scopus |
| PMCID: | PMC7988237 |
| PUBMED: | 33786456 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 April 2021 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work