Transformation of rat fibroblasts by FSV rapidly increases glucose transporter gene transcription Journal Article
| Authors: | Birnbaum, M. J.; Haspel, H. C.; Rosen, O. M. |
| Article Title: | Transformation of rat fibroblasts by FSV rapidly increases glucose transporter gene transcription |
| Abstract: | Elevation of glucose transport is an alteration common to most virally induced tumors. Rat fibroblasts transformed with wild-type or a temperature-sensitive Fujinami sarcoma virus (FSV) were studied in order to determine the mechanisms underlying the increased transport. Five- to tenfold increases in total cellular glucose transporter protein in response to transformation were accompanied by similar increases in transporter messenger RNA levels. This, in turn, was preceded by an absolute increase in the rate of glucose transporter gene transcription within 30 minutes after shift of the temperature-sensitive FSV-transformed cells to the permissive temperature. The transporter messenger RNA levels in transformed fibroblasts were higher than those found in proliferating cells maintained at the nonpermissive temperature. The activation of transporter gene transcription by transformation represents one of the earliest known effects of oncogenesis on the expression of a gene encoding a protein of well-defined function. |
| Keywords: | nonhuman; animal cell; animal; cell division; heredity; cell line; genetic transcription; transcription, genetic; gene expression regulation; kinetics; rna, messenger; rat; fibroblast; fibroblasts; rats; glucose transport; cell transformation, viral; glucose transporter; monosaccharide transport proteins; priority journal; virus cell transformation; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; sarcoma viruses, avian; fujinami sarcoma oncovirus |
| Journal Title: | Science |
| Volume: | 235 |
| Issue: | 4795 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 1987-03-20 |
| Start Page: | 1495 |
| End Page: | 1498 |
| Language: | English |
| DOI: | 10.1126/science.3029870 |
| PUBMED: | 3029870 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
