Expression of a functional influenza viral cap-recognizing protein by using a bovine papilloma virus vector Journal Article
| Authors: | Braam-Markson, J.; Jaudon, C.; Krug, R. M. |
| Article Title: | Expression of a functional influenza viral cap-recognizing protein by using a bovine papilloma virus vector |
| Abstract: | The gene for the influenza viral PB2 protein, which recognizes and binds the 5'-terminal cap 1 structures (m7GpppNm) on eukaryotic mRNAs, was inserted into a bovine papilloma virus vector under the control of a mouse metallothionein I (MT-I) promoter. After transfection of this vector into mouse NIH 3T3 cells, a cell line, cPB2, was obtained that produces PB2-specific mRNA and authentic PB2 protein. Induction of the MT-I promoter with CdCl2 causes about a 10-fold increase in PB2 mRNA and protein levels. The expressed PB2 protein is functional, as it relieves the block in viral mRNA synthesis exhibited by a temperature-sensitive viral mutant containing a cap-binding defect in the PB2 protein. This demonstrates complementation of a function of a negative-strand RNA virus by a gene product expressed in a cell line from recombinant DNA. |
| Keywords: | nonhuman; mouse; gene expression; in vitro study; genetic transfection; genetic engineering; cell culture; eukaryota; rna viruses; radioisotope; virus recombinant; polyacrylamide gel electrophoresis; influenza virus; papilloma virus; bovinae; papillomavirus; priority journal; bovine papilloma virus |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 82 |
| Issue: | 13 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1985-07-01 |
| Start Page: | 4326 |
| End Page: | 4330 |
| Language: | English |
| DOI: | 10.1073/pnas.82.13.4326 |
| PROVIDER: | scopus |
| PMCID: | PMC390406 |
| PUBMED: | 2989815 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |
