In vitro evidence for genetic predisposition in some human pancreatic adenocarcinoma Journal Article
| Authors: | Shannon Danes, B.; Boyle, P. D.; Traganos, F.; Melamed, M. R. |
| Article Title: | In vitro evidence for genetic predisposition in some human pancreatic adenocarcinoma |
| Abstract: | A numerical alteration in chromosome complement in human dermal fibroblast cultures, hyperdiploidy with a normal occurrence of tetra-ploidy (IVH) has been reported to be associated with some hereditary single tumors, including pancreatic adenocarcinoma (PaCa). The incidence of IVH was compared in cultures derived from 34 PaCa patients and 39 clinically normal subjects without a family tumor history by three assay systems: (a) percentage of numerically altered metaphases in chromosome preparations (MA); (b) percentage of cells from high-density, stationary cultures with a DNA index (DI) greater than 1 (FCMS); (c) percentage of cells with a DI greater than 2 from cultures of cells undergoing division (FCMd). The last two measurements were obtained by flow cytometric measurement of propidium idodide (PI) stained cells. Concordance was observed between the three assays. There was a linear relationship between the percentage of hyperdiploid metaphases assayed in the chromosome preparation and the percentage of cells with a DI > 1 by FCMSand a DI > 2 by FCMd. IVH was considered to be present (IVH+) by the MA technique if the percentage of numerically altered metaphases was over 4% exclusive of tetraploids, by the FCMS technique if DI > 1 was > 10%, and by FCMdif DI > 2 was > 7%. The assayed group could be divided into two IVH groups on the basis of all three assays, individually or collectively. The IVH - group consisted of all the normal assayed (39/39) and 71% (24/34) of the PaCa patients, and the IVH+group the remaining 29% (10/34) of the PaCa patients. The average age of PaCa diagnosis was earlier (mean 42 years old) for the IVH+group than for the IVH-PaCa group (mean 75 years old). All three assays were done to assign individual IVH status in this study. These data gave a predictive value for IVH (based on its specificity and sensitivity), which may ultimately have practical value for identification of genetic predisposition for this low prevalence tumor. © 1987 Raven Press, New York. |
| Keywords: | adolescent; adult; aged; aged, 80 and over; human cell; pancreas cancer; pancreatic neoplasms; flow cytometry; adenocarcinoma; cytology; tumor cells, cultured; histology; cell culture; dna, neoplasm; pancreatic adenocarcinoma; tetraploidy; metaphase; fibroblast culture; diploidy; in vitro; middle age; disease susceptibility; human; male; female; priority journal; support, non-u.s. gov't; in vitro hyperdiploidy (ivh); cultured human dermal fibroblast; flow cytometry assays; ivh assays: metaphase assay (ma) |
| Journal Title: | Pancreas |
| Volume: | 2 |
| Issue: | 5 |
| ISSN: | 0885-3177 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1987-09-01 |
| Start Page: | 584 |
| End Page: | 588 |
| Language: | English |
| DOI: | 10.1097/00006676-198709000-00015 |
| PUBMED: | 3671349 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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