Changes in cell surface glycoprotein expression during differentiation of human keratinocytes Journal Article

  


Authors: Klein, C. E.; Cordon-Cardo, C.; Soehnchen, R.; Cote, R. J.; Oettgen, H. F.; Eisinger, M.; Old, L. J.
Article Title: Changes in cell surface glycoprotein expression during differentiation of human keratinocytes
Abstract: Six cell surface glycoproteins defined by monoclonal antibodies were selected for study on human epidermal cells. In tests on tissue sections, three of the glycoproteins [ J143 (gp 140 30); T43 (gp 85 36); H99 (gp38)] were expressed in the basal cell layer of the epidermis, whereas the other three glycoproteins [T179 (gp 140 95); T16 (gp 40 50); BT15 (gp80)] were preferentially expressed in maturing keratinocytes above the basal layer. We compared synthesis of these glycoproteins in fresh epidermis and in primary epidermal short term cultures using [35S]methionine for metabolic labeling. Synthesis of J143 was 8- to 20-fold higher and synthesis of T43 was 4- to 10-fold lower in cultured cells compared with fresh epidermis. BT15, an antigen strongly expressed on terminally differentiating keratinocytes, was synthesized at 5- to 15-fold higher levels in fresh epidermis than in cultured cells. Biosynthesis levels of H99, T179, and T16 did not change in cultured epidermal cells. Based on our findings, we propose a model of surface antigenic changes that occur during keratinocyte differentiation in vivo. © 1987.
Keywords: immunohistochemistry; adult; human cell; cytology; cells, cultured; epidermis; membrane proteins; cell differentiation; keratinocyte; monoclonal antibody; gene expression regulation; antibodies, monoclonal; glycoproteins; glycoprotein; normal human; human; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.
Journal Title: Journal of Investigative Dermatology
Volume: 89
Issue: 5
ISSN: 0022-202X
Publisher: Elsevier Science, Inc.  
Date Published: 1987-11-01
Start Page: 500
End Page: 506
Language: English
DOI: 10.1111/1523-1747.ep12460996
PUBMED: 3668293
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023243151&doi=10.1111%2f1523-1747.ep12460996&partnerID=40&md5=ce6bb6aa2674be519bc62d760b85816c
Notes: Article -- Export Date: 5 February 2021 -- Source: Scopus
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MSK Authors
  1. Carlos Cordon-Cardo
    612 Cordon-Cardo
  2. Herbert F Oettgen
    130 Oettgen
  3. Lloyd J Old
    593 Old
  4. Richard J. Cote
    27 Cote
  5. Magdalena G. Eisinger
    25 Eisinger
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