| Abstract: |
Treatment of human cells with interferon-α transiently increases the rate of transcription of mRNA 561, which encodes a 56-kD protein of unknown function. Using a cDNA probe specific for this mRNA, we determined the chromosomal localization of the corresponding gene. Southern blot analyses of genomic DNA samples isolated from 19 independent mouse-human and hamster-human cell hybrids, each of which contained all rodent chromosomes and several human chromosomes, indicated that this gene is located on human chromosome 10. The same conclusion was drawn independently from studies using in situ hybridization of the probe to human metaphase chromosomes. Furthermore, the latter approach enabled us to conclude that the gene is situated most likely at the junction of 10q25-q26. © 1987, SDAMAL. All rights reserved. |
| Keywords: |
human cell; nonhuman; alpha interferon; mouse; animal; mice; heredity; genetic transcription; transcription, genetic; in vitro study; dna; drug mechanism; genetic engineering; rna, messenger; nucleic acid hybridization; gene location; chromosome mapping; chromosome 10q; interferon type i; hybrid cell; hybrid cells; hamster; chromosomes, human, pair 10; human; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.; hamsters
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