Analysis of prostaglandin E(2) effect on T lymphocyte activation: Abrogation of prostaglandin E(2) inhibitory effect by the tumor promoter 12.0 tetradecanoyl phorbol-13 acetate Journal Article

   

Authors:	Chouaib, S.; Robb, R. J.; Welte, K.; Dupont, B.
Article Title:	Analysis of prostaglandin E(2) effect on T lymphocyte activation: Abrogation of prostaglandin E(2) inhibitory effect by the tumor promoter 12.0 tetradecanoyl phorbol-13 acetate
Abstract:	We have investigated the inhibitory potential of prostaglandin E2 (PGE2) with respect to intracellular messengers implicated in the signaling system of T-lymphocyte activation pathway. Using the fluorescent indicator Quin 2, it is demonstrated that PGE2 inhibits the increase in cytosolic-free calcium concentration [CA2+](i). Reconstitution of calcium mobilization in the presence of PGE2 by the calcium ionophore A23187 results in a partial restoration of both interleukin 2 (IL2) production and cell proliferation and has no effect on the inhibition of transferrin receptor expression. In contrast, the treatment of cell cultures with the tumor promoter 12.0 tetra decanoyl phorbol-13-acetate (TPA) abrogates the suppressor activity of PGE2. When T lymphocyte stimulation is provided by the combination of A23187 and TPA, the PGE2 inhibitory effect does not occur. These data also indicate that the down regulation of transferrin receptor by PGE2 is proximal to protein kinase C activation and is not associated with decreased expression of the functional IL2 receptor.
Keywords:	t lymphocyte; t-lymphocytes; interleukin 2; calcium; enzyme activation; in vitro study; drug effect; physiology; immunological tolerance; lymphocyte activation; immune tolerance; prostaglandin e2; cytoplasm; cyclic amp; protein kinase c; prostaglandin e; prostaglandins e; interleukin-2; in vitro; receptors, immunologic; phorbol 13 acetate 12 myristate; tetradecanoylphorbol acetate; dinoprostone; interleukin 2 receptor; receptors, interleukin-2; transferrin receptor; immunoglobulin receptor; receptors, transferrin; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; calcimycin; receptors, interleukin 2; quin 2
Journal Title:	Journal of Clinical Investigation
Volume:	80
Issue:	2
ISSN:	0021-9738
Publisher:	American Society for Clinical Investigation
Date Published:	1987-08-01
Start Page:	333
End Page:	340
Language:	English
DOI:	10.1172/jci113077
PUBMED:	3038954
PROVIDER:	scopus
PMCID:	PMC442242
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023160915&doi=10.1172%2fJCI113077&partnerID=40&md5=0be48c3052c3bcc93771983d5149ed2a
Notes:	Article -- Export Date: 5 February 2021 -- Source: Scopus

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