The response of human marrow colony-forming units-granulocyte and macrophage to inhibition by prostaglandin E and acidic isoferritins is associated with expression of MHC class II antigens and requires the participation of a CD8(+) T lymphokine Journal Article
| Authors: | Pelus, L. M.; Levi, E.; Welte, K. |
| Article Title: | The response of human marrow colony-forming units-granulocyte and macrophage to inhibition by prostaglandin E and acidic isoferritins is associated with expression of MHC class II antigens and requires the participation of a CD8(+) T lymphokine |
| Abstract: | The in vitro expression of MHC class II Ag by human bone marrow colony-forming unit-granulocyte and macrophage (CFU-GM) and their proliferative response to negative growth regulators in agar culture are transient and can be modulated in vitro by 24-h suspension culture in the presence of PGE. Analysis of the participation of accessory cells in this phenomenon indicates that the ability of PGE to modulate CFU-GM MHC class II expression, the proportion of CFU-GM in S-phase of the cell cycle and the responsiveness of CFU-GM to inhibition in vitro by two negative growth regulators, acidic isoferritin inhibitory activity and PGE itself, requires the participation of CD8+ T lymphocytes. This effect is mediated by a lymphokine of m.w. 27,000 that we have purified to apparent homogeneity. This lymphokine possesses neither colony stimulatory nor inhibitory activity, is produced by both peripheral blood and bone marrow CD8+ T lymphocytes, as well as the CEM T-ALL cell line, and requires the obligatory presence of PGE for activity. |
| Keywords: | adult; human cell; cd8 antigen; t lymphocyte; t-lymphocytes; phenotype; bone marrow cells; cell cycle; bone marrow; cell line; cell culture; major histocompatibility antigen class 2; hematopoietic stem cells; macrophage; macrophages; hla-d antigens; prostaglandin e; prostaglandins e; cell separation; antigen-presenting cells; granulocyte; normal human; ferritin; culture media; hla system; colony forming unit; growth inhibitors; granulocytes; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; lymphokine; suppressor factors, immunologic; acid isoferritin |
| Journal Title: | Journal of Immunology |
| Volume: | 141 |
| Issue: | 5 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1988-09-01 |
| Start Page: | 1658 |
| End Page: | 1664 |
| Language: | English |
| PUBMED: | 3261758 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
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