Defining conditions to promote the attachment of adult human colonic epithelial cells Journal Article

  


Authors: Buset, M.; Winawer, S.; Friedman, E.
Article Title: Defining conditions to promote the attachment of adult human colonic epithelial cells
Abstract: An improved method for the attachment and growth of normal human colonic epithelial cells from minute 1 to 3-mm3 biopsies has been developed. This yields four times as many cultured cells per biopsy than older methods, with a success rate of 97% in a series of 29 biopsies. Fetal bovine serum was eliminated from the medium, the medium pH was decreased to 6.7, the oxygen tension in the incubator was decreased to 3%, and the NCTC 168 medium was supplemented with ethanolamine, phosphoethanolamine, hydrocortisone, ascorbic acid, transferrin, glutamine, insulin, epidermal growth factor, pentagastrin, and deoxycholic acid. The best substrate for cell attachment was a mixture of ungelled collagen I and bovine serum albumin. This substrate was better than the identical mixture with fibronectin added, fibronectin alone, a thin gelatin film, collagen IV with or without fibronectin, and basement membrane preparations from four different cell lines. © 1987 Tissue Culture Association, Inc.
Keywords: comparative study; cytology; cells, cultured; cell division; basement membrane; oxygen; culture medium; ph; biopsy; physiology; cell culture; collagen; epithelium cell; epithelial cells; bovine serum albumin; serum albumin, bovine; cell adhesion; colon; hydrogen-ion concentration; culture media; fibronectin; fibronectins; human; article; normal colonic epithelial cell culture
Journal Title: In Vitro Cellular & Developmental Biology
Volume: 23
Issue: 6
ISSN: 0883-8364
Publisher: Tissue Culture Association  
Date Published: 1987-06-01
Start Page: 403
End Page: 412
Language: English
DOI: 10.1007/bf02623855
PUBMED: 3597281
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023353748&doi=10.1007%2fBF02623855&partnerID=40&md5=80db81975e3a755d0966a1529c746fe6
Notes: Article -- Export Date: 5 February 2021 -- Source: Scopus
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MSK Authors
  1. Sidney J Winawer
    274 Winawer
  2. Eileen A. Friedman
    70 Friedman
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